FAQ: Can a lysis buffer other than the Luciferase Cell Lysis Buffer be used to make cell lysates for assaying GLuc (or CLuc) acitivity?

No. Other lysis buffers may contain components that can interfere with the BioLux GLuc or CLuc assay systems. For assaying GLuc or CLuc activity from cell lysates the use of the Luciferase Cell Lysis Buffer to generate those lysates is recommended.