A growing number of methods are being developed and shared by the scientific community around the world for use in COVID-19-related work, some of which have been approved as FDA EUA protocols. A selection of preprints and protocols is shown below. Please note that preprints, protocols, and early access publications have not completed a peer-review process.

LAMP: Colorimetric and Fluorescence Detection Methods


Clinical assessment and validation of a rapid and sensitive SARS-CoV-2 test using reverse-transcription loop-mediated isothermal amplification
Anahtar, M. et. al.
Massachusetts General Hospital, Harvard Medical School, New England Biolabs, Howard Hughes Medical Institute, Blavatnik Institute

This preprint presents a simple clinical workflow that utilizes a sensitive and highly specific colorimetric RT-LAMP assay to detect SARS-CoV-2 in a 40-minute protocol.



Rapid and extraction-free detection of SARS-CoV-2 from saliva with colorimetric LAMP
Lalli, M. et. al.
Washington University School of Medicine

This preprint outlines development of a rapid colorimetric assay using RT-LAMP optimized on human saliva samples without an RNA purification step.



Host, Viral, and Environmental Transcriptome Profiles of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
Butler, D.J. et al.
Weill Cornell Medicine, Baylor College of Medicine, Hudson Alpha Discovery Institute, University Hospital Tuebingen, Columbia University, New England Biolabs

This preprint outlines the design of a 30-minute colorimetric LAMP test to identify SARS-CoV-2 infection and development of a large-scale shotgun metatranscriptomic profiling platform for nasopharyngeal swabs.



SARS-CoV-2 On-the-Spot Virus Detection Directly from Patients
Nadav, B. et. al.
Technion – Israel Institute of Technology, Meir Medical Center, Tel-Aviv University, Canadian Institute for Advanced Research

This preprint describes development of a protocol based on RT-LAMP for direct detection of SARS-CoV-2 on nose and throat swabs, with no RNA purification step required.



Point-of-care testing for COVID-19 using SHERLOCK diagnostics
Joung, J. et. al.
Howard Hughes Medical Institute, Broad Institute, MIT, McGovern Institute, Massachusetts General Hospital, Brigham and Women’s Hospital, Ragon Institute, University of Washington, Fred Hutchinson Cancer Research Center

The preprint outlines a simple test chemistry called STOP (SHERLOCK Testing in One Pot) for detecting SARS-CoV-2 in one hour that is suitable for point-of-care use. This simplified test, STOPCovid, provides sensitivity comparable to RT-qPCR-based SARS-CoV-2 tests and has a limit of detection of 100 copies of viral genome input in saliva or nasopharyngeal swabs per reaction.



EasyCOV : LAMP based rapid detection of SARS‐CoV‐2 in saliva
L’Helgouach et. al.
Sys2Diag, Montpellier University Hospital

This preprint describes a direct saliva RT-LAMP based virus detection assay called EasyCOV that utilizes a colorimetric readout of test results.



FDA EUA: Color Genomics, Inc. SARS CoV-2 Diagnostic Assay

Luna RT-qPCR


Direct RT-qPCR Detection of SARS-CoV-2 RNA From Patient Nasopharyngeal Swabs Without an RNA Extraction Step
Bruce, E. et. al.
University of Vermont, University of Washington, Fred Hutchinson Cancer Research Center, IXIS LLC

This preprint describes a direct RT-qPCR detection assay that omits the RNA extraction step. In a blinded study, the direct assay correctly identified 92% of nasopharyngeal samples demonstrated to be positive for SARS-CoV-2 RNA by traditional methods that included RNA extraction.



A blueprint for the implementation of a validated approach for the detection of SARS-Cov2 in clinical samples in academic facilities
Sridhar, S. et. al.
University of Cambridge, Wellcome Sanger institute, Addenbrookes Hospital

This preprint describes the process of developing a SARS-CoV-2 diagnostic workflow in a conventional academic Containment Level 2 (CL2) laboratory using RT-qPCR detection.



Optimized qRT-PCR approach for the detection of intra- and extra-cellular SARS-CoV-2 RNAs
Toptan, T. et. al.
Goethe University, GenXPro Gmbh

This preprint outlines SARS-CoV-2 detection that can be accomplished with either probe or dye-based RT-qPCR approaches.



Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
Waggoner, JJ. et. al.
Emory University, Stanford University

This research letter describes development of a triplex assay for streamlined detection of the N2, E, and RNase P targets in SARS-CoV-2 RNA.



FDA EUA: Diatherix Eurofins Laboratory SARS-CoV-2 PCR Test


FDA EUA: InBios Smart Detect SARS-CoV-2 rRT-PCR Kit

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Welcome to the COVID-19 researcher spotlight

Hear from scientists around the world as they discuss publications related to COVID-19, new protocols and techniques for detection and characterization, as well as how the scientific community is coming together to address this pandemic...all in 19 minutes or less!

COVID-19 Rabe Podcast

In this episode of the NEB podcast, Lessons from Lab and Life, hear from Brian Rabe of Harvard Medical School about his work applying RT-LAMP to develop a fast SARS-CoV-2 diagnostic assay.

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