RT-qPCR, RT-PCR and cDNA Synthesis
RNA can be detected, quantified and manipulated through copying via the use of reverse transcriptases (RTs), which are RNA-dependent DNA polymerases. RTs synthesize a strand of DNA that is complementary to the original RNA template and is referred to as cDNA. This cDNA can then be further amplified through PCR, qPCR, or isothermally using, for example, one-step RT-LAMP. Alternately, the cDNA can be used in library preparation for next generation sequencing.
New England Biolabs provides a broad variety of reverse transcriptases and kits for use in these experiments. In addition to stand alone enzymes such as ProtoScript II, NEB provides a number of optimized kits for RT-qPCR, RT-PCR, RNA Seq and isothermal RNA detection.
Choose Type:
- cDNA synthesis on oligo (dT)25 magnetic beads (S1419)
- First Strand cDNA Synthesis Protocols (E6300)
- Isolation of mRNA Using the PolyA Spin™ mRNA Isolation Kit
- First strand cDNA synthesis OneTaq® RT-PCR Kit
- PCR Amplification with OneTaq® RT-PCR Kit
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0277)
- First Strand cDNA Synthesis Protocols (E6560)
- Protocol for Avoiding Rnase Contamination using Murine Rnase Inhibitor (M0314)
- Protocol for Avoiding Rnase Contamination using Rnase Inhibitor, Human Placenta (M0307)
- One-Step RT-PCR Protocols (E5315)
- Typical cDNA Synthesis Protocol
- Typical RT-LAMP Protocol
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0277)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0277)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0253)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0368)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0253)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0368)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0253)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0368)
- Luna Universal qPCR And RT-qPCR
- PCR Brochure
- RNA Technical Guide
- Avoiding Ribonuclease Contamination
Brochures
Usage Guidelines
- Potapov, V., Fu, X., Dai, N., Correa, I.R., Jr., Tanner, N.A., Ong, J.L 2018. Base modificatons affecting RNA polymerase and reverse transcriptase fidelity Nucleic Acids Research. 46(11): 5753-5763, PubMedID: 29750267, DOI:
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Videos
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“Dots in Boxes" Visualization of qPCR Data
Learn how we analyzed plates and plates of qPCR data, and how Luna stacks up to the competition.
