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RNA Modification

RNA can be modified with enzymes and reagents that act selectively depending on the existence of particular structures, and hence are useful tools for characterizing RNA species. The selective properties of RNA modifying enzymes enable researchers to distinguish different RNA species. Some unique end modifications can be used to selectively degrade or isolate particular RNA species when treated sequentially with the appropriate series of enzymes. For instance, 5´-capped RNAs are not substrates for polynucleotide kinases, or RNA ligases, but are substrates for decapping enzymes. Similarly, ligation and poly-adenylation of RNA 3´ end requires a free 3´.

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Protocols for RNA Modification
    Tools & Resources

    Feature Articles

  • Mind your caps and Poly A tails

    Understand options for selecting reagents and the extent of reagent influence on synthesized mRNA cap functionality.

    • Brochures

  • Latest NEB Expressions

    This edition of NEB expressions discusses breakthroughs in Golden Gate Assembly research, that now enables 20+ fragment assembly with high efficiency and accuracy. New products include the NEBNext® Single Cell/Low Input RNA Library Prep Kit and the NEBNext Direct® Custom Ready Panels for NGS Target Enrichment. Lastly, learn about the importance of basic research at NEB in an interview with NEB’s Research Director.

  • RNA Synthesis Brochure

    Learn more about NEB's products for RNA synthesis, which range from template generation to poly(A) tailing.

  • RNA Technical Guide

    Find in depth information on tools designed to streamline your RNA workflows, including RNA extraction & purification, qPCR and RT-qPCR, RNA-seq, RNA synthesis and gRNA synthesis.

    • Usage Guidelines

  • Avoiding Ribonuclease Contamination
RNA Ligase Selection Chart
NEB offers a variety of ligases for RNA research with unique specificities.
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While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Videos

  1. invitro_gaussia_video_tn

    In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

    This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. 

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