RNA is a polynucleotide that is typically transcribed from a DNA template. Historically, its only known function was involvement in the translation of genetic information into proteins, either through encoding the sequence of the protein (messenger RNA; mRNA), bringing amino acids to the ribosome (transfer RNA; tRNA), or by being a component of the ribosome translational machinery (ribosomal RNA; rRNA). Recently, technological advances and innovative research in the field of RNA analysis, have led to the discovery of many new classes of small and large non-coding RNAs with novel regulatory functions. These findings have helped usher in a renaissance of RNA-focused research in biology.
RNA Analysis includes these areas of focus:
Protocols for RNA Analysis
Learn more about NEB's products for RNA, which range from template generation to poly(A) tailing.
- Recommended HiScribe RNA Synthesis Kits by Application
- Troubleshooting Guide for RNA Purification
- Avoiding Ribonuclease Contamination
- Guidelines for Purification of RNA from Cultured Mammalian Cells
Other Tools & Resources
- Fuchs, R.T., Sun, Z., Zhuang, F., et al. 2015. Bias in ligation-based small RNA sequencing library construction is determined by adaptor and RNA structure PLoS One. 10(5), PubMedID: 25942392, DOI: 10.1371/journal.pone.0126049
Publications related to RNA Analysis
RNA Reagents Overview
Reagents for RNA Sample Preparation
RNA Polymerase Selection Chart
RNA Ligase Selection Chart
cDNA Synthesis Selection Chart
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This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene.