RNA Analysis

Historically the function of RNA in the cell was to assist in the translation of genetic information from DNA into protein. The major species of RNA were; (A) messenger RNA, which converts the genetic information of DNA into RNA, (B) transfer RNA (tRNA) that are charged with specific amino acids and, (C) ribosomal RNA (rRNA), a major component of the ribosome.  RNA has now been implicated in a diverse number of biological processes including catalysis and transcriptional regulation. Recently, technological advances and improvements in RNA analysis and detection have led to the discovery of many new classes of small and large non-coding RNAs with novel regulatory functions.  Examples include, microRNA (miRNA), circular RNA, long  non-coding RNA (lncRNA), small nucleolar RNA (snoRNA) and extracullular RNA (exRNA). In addition, RNA modifications have revealed added complexity to RNA. These biologically relevant modifications are an active area of exploration. These findings have helped usher in a renaissance of RNA-focused research in biology.

RNA Analysis

RNA Analysis includes these areas of focus:

In vitro Synthesis (IVT)
RNA Labeling
RNA Modification
RNA Purification and Isolation
RT-qPCR, RT-PCR and cDNA Synthesis
Small RNA Detection and Isolation

    Publications related to RNA Analysis

  1. Fuchs, R.T., Sun, Z., Zhuang, F., et al. 2015. Bias in ligation-based small RNA sequencing library construction is determined by adaptor and RNA structure PLoS One. 10(5), PubMedID: 25942392, DOI: 10.1371/journal.pone.0126049

Recommended HiScribe RNA Synthesis Kits by Application

RNA Polymerase Selection Chart

NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.

RNA Ligase Selection Chart

NEB offers a variety of ligases for RNA research with unique specificities.

cDNA Synthesis Selection Chart

NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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    NEBNext Ultra II Directional RNA Workflow

    Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.

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    In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

    This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene.