The NEBExpress® Cell-free E. coli Protein Synthesis System is a lysate-based coupled transcription/translation system designed to synthesize proteins encoded by a DNA template under the control of a T7 RNA Polymerase promoter. The system offers high expression levels, the ability to produce high molecular weight proteins, scalability, and is cost-effective for high throughput expression applications. The speed and robustness of the system facilitates protein synthesis in applications such as protein engineering, mutagenesis studies and enzyme screening. In addition, it can be used to generate proteins for biophysical and structure-function analyses.
Protein synthesis is achieved using a short incubation and the synthesized protein is compatible with downstream purification or analysis by SDS-PAGE, Western Blot or direct functional assay. The novel formulation of this system allows samples to be loaded directly onto SDS-PAGE, without the need for acetone or TCA precipitation. Additionally, the synthesized protein can be isolated from the reaction mixture by affinity purification techniques such as immobilized metal affinity chromatography (IMAC) for further structural and/or functional characterization.
The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
- Protein Expression & Purification Brochure
- Purification Beads, Columns and Resins Brochure
- PURExpress® vs. NEBExpress® Application Chart
- Protein Expression and Purification Selection Chart
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Watch this tutorial explaining the streamlined workflow for our new NEBExpress® Cell-free Protein Synthesis System to learn how you can easily synthesize your protein in as little as 2 to 4 hours.