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Protein Labeling

The SNAP-tag®, CLIP-tag™, and ACP-tag/MCP-tag protein labeling technologies offer an innovative alternative to traditional fluorescent proteins for studying the function and localization of proteins in living and fixed cells. Covalent protein labeling brings simplicity and versatility to the imaging of mammalian proteins in live cells, as well as the ability to capture proteins in vitro. The creation of a single genetic construct generates a fusion protein which, when covalently attached to a variety of substrates, including fluorophores, biotin, and beads, provides a powerful tool for studying proteins. In the SNAP-tag and CLIP-tag sytems, the fusion protein is labeld by a self-labeling tag based on the DNA repair protein O6-alkylguanine-DNA-alkyltrasferase, whereas with the ACP-tag and MCP-tag systems, the labeling is catalyzed through a post-translational modification by a phosphopantetheinyl transferase.

Another way of labeling proteins is by using intein-mediated protein ligation (1) or expressed protein ligation. The IPL reaction allows the ligation of a synthetic peptide or a protein with an N-terminal cysteine residue to the thioester on the C-terminus of an expressed protein through a native peptide bond. The IPL protocol employs an IMPACT™ (NEB #E6901) C-terminal fusion vector to express and purify a protein of interest and to generate a thioester at its C-terminus. IPL allows for ligation of peptides (may contain modified amino acids) or proteins to the C-terminus of an IMPACT expressed protein.

  

  1. Evans, T. et al. (1998) Protein Sci. 7, 2256-2264. PMID: 9827992 
  2. Muir, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95,6705-6710. PMID: 9618476

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Protein Labeling includes these areas of focus:
Peptide Ligation
Protein Labeling (SNAP/CLIP)
FAQs for Protein Labeling
Protocols for Protein Labeling
Application Notes for Protein Labeling
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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