The standard workflow for DNA sequencing on an Ion Torrent™ sequencer begins with end repair of the 3´and 5´ends, adaptor ligation, size-selection of adaptor-ligated molecules, and PCR amplification. Refer to the DNA Preparation Workflow for Ion Torrent tab below for a graphic illustration of the steps.
Once the adaptor-ligated molecules are amplified, they are ready for template preparation, the next step towards Ion Torrent sequencing.
NEBNext® reagents are available for each of the above described steps, including the fragmentation step. All NEBNext products are functionally validated, both as single reagents and by generating an Ion Torrent library. For details on typical data obtained with NEBNext reagents, refer to the “Representative results” tabs below.
Ion Torrent® is a trademark owned byThermo Fisher Scientific.
- NEBNext FFPE DNA Repair Mix (M6630) - Protocol for use with Other User-supplied Library Construction Reagents
- Protocol for a PCR reaction using NEBNext® Q5® Hot Start HiFi PCR Master Mix (M0543)
- Protocol for use with NEBNext FFPE DNA Repair Mix (M6630) and NEBNext DNA Library Prep Master Mix Set for Illumina (E6040)
- Protocol for use with NEBNext Ultra DNA Library Prep Kit for Illumina (E7370)
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