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FFPE DNA Repair

Formalin, a formaldehyde-based solution, is commonly used as a fixative for the long-term storage of tissue samples. The mechanism by which formalin prevents sample degradation is through the formation of several different types of chemical interactions between adjacent macromolecules within the tissue sample – including between and within DNA molecules. After fixation with formalin, the tissue sample is usually embedded in paraffin; the paraffin both promotes tissue preservation and provides a scaffold for tissue sectioning.

While the fixation process adequately preserves the ultrastructure of the tissues, it results in various types of damage to the DNA within the tissues. The DNA damage signature of FFPE DNA includes:
  • Hydrolysis of N-glycosyl bond
    • ~ 0.5 depurination events yr-1 kb-1 (depyrimidation occurs at 5% of this rate). Rate significantly enhanced by lower pH.
  • Deamination
    • Mostly cytosine to uracil (~ 1 deamination yr-1 kb-1 for ssDNA and ~0.005 yr-1 kb-1 for dsDNA).
  • Oxidation
    • 8-hydroxyguanine formation
    • Thymine or cytosine glycol formation
  • Thymine dimers
  • Nicks
  • Double strand breaks

Gathering meaningful biochemical data, including high-quality DNA sequence information, from these samples reproducibly poses a challenge. An additional challenge to analysis of archived tissue samples is that the typical sample is generally small and may only contain trace amounts of the tissue of interest and, therefore, the nucleic acid of interest. For these reasons, studies based on sequencing of FFPE samples have been technically challenging. Below is an example of a sample block of formalin-fixed, paraffin-embedded tissue.



 
  1. Lindahl, T., (1993) Nature, 362:709

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FFPE DNA Repair includes these areas of focus:
NEBNext FFPE DNA Repair Mix
Protocols for FFPE DNA Repair
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While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

  1. Behind the paper: DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification

    Behind the paper: DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification

    NEB researchers published a paper in Science highlighting DNA damage as a prevalent source of errors in public cancer databases. Learn about how addressing this damage could improve the detection of low-frequency disease variants.

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