DNA Methylation Analysis

Methods to analyze DNA methylation, DNA demethylation, and their functional effects are critical to epigenetics researchers. Methylation profiles of epigenome are used for disease identification and for research and therapeutic development. Analysis can be gene-specific or global depending on downstream applications (1).

Downstream methylation detection methods include end-point PCR, real-time PCR, primer extension, single-stranded conformational polymorphism assays, blotting, microarray analysis and sequencing. Choosing which method(s) to use largely depends on the experimental sample size and the goals of the experiments.

  1. Tao Zuo et al. 2009, Epigenomics  1(2) 331-345. PMID: 20526417

Choose Type:

DNA Methylation Analysis includes these areas of focus:
5-hmC Detection & Analysis
Bisulfite Conversion
Methylated DNA Analysis
FAQs for DNA Methylation Analysis
Protocols for DNA Methylation Analysis
Application Notes for DNA Methylation Analysis
    Publications related to DNA Methylation Analysis
  1. Woodcock, C.B.l, Yu, D., Hajian, T., Li, J., Huang, Y., Dai, N., Corrêa, I.R., Jr., Wu, T., Vedadi, M., Zhang, X., Cheng, X 2019. Human MettL3 MettL14 complex is a sequence specific DNA adenine methyltransferase active on single strand and unpaired DNA in vitro Cell Discov . 5:63, PubMedID: 31885874, DOI: 10.1038/s41421-019-0136-4
  2. Pirone-Davies, C., Hoffmann, M., Roberts, R.J., Muruvanda, T., Timme, R.E., Strain, E., Luo, Y., Payne, J., Luong, K., Song, Y., Tsai, Y.C., Boitano, M., Clark, T.A., Korlach, J., Evans, P.S., Allard, M.W. 2015. Genome-Wide Methylation Patterns in Salmonella enterica Subsp. enterica Serovars PLoS One. 10(4), PubMedID: 25860355, DOI:
  3. Pais, J.E., Dai, N., Tamanaha, E., Vaisvila, R., Fomenkov, A.I., Bitinaite, J., Sun, Z., Guan, S., Correa, I.R. Jr., Noren, C.J., Cheng, X., Roberts, R.J., Zheng, Y. and Saleh, L. 2015. Biochemical characterization of a Naegleria TET-like oxygenase and its application in single molecule sequencing of 5-methylcytosine Proc Natl Acad Sci U S A. 112(14), PubMedID: 25831492, DOI:
  4. Hughes J.R., Roberts N., McGowan S., Hay D., Giannoulatou E., Lynch M., De Gobbi M., Taylor S., Gibbons R., Higgs D.R. 2014. Analysis of hundreds of cis-regulatory landscapes at high resolution in a single, high-throughput experiment Nat Genet. 46 (2), PubMedID: 24413732, DOI: doi:10.1038/ng.2871
  5. Sexton T, Kurukuti S, Mitchell JA, Umlauf D, Nagano T, Fraser P 2012. Sensitive detection of chromatin coassociations using enhanced chromosome conformation capture on chip Nat Protoc. 7(7), PubMedID: 22722369, DOI: 10.1038/nprot.2012.071
Methods of DNA Methylation Analysis
  • Bisulfite conversion, which provides single nucleotide, high resolution data for whole genomes or single locus methylation studies.
  • Differential enzymatic cleavage, which uses methylation, or dually 5-mC and 5-hmC dependent enzymes, paired with non-sensitive isoschizomers to asses DNA methylation or DNA hydroxymethylation.
  • High-Performance Liquid Chromatography (HPLC)
  • Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry(MALDI-TOF MS)
  • Methylated DNA-ELISA
  • Affinity-based methods, which use methylated DNA binding proteins or antibodies for enrichment.
  • Enzymatic DNA methyltransferase assay
Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.