DNA Amplification

Hot Start PCR

Hot Start PCR is a technique that reduces non-specific amplification and offers the convenience of reaction set up at room temperature. The polymerases used in Hot Start PCR are unreactive at ambient temperatures. Polymerase activity can be inhibited at these temperatures through different mechanisms, including antibody interaction, chemical modification and aptamer technology. At permissive reaction temperatures reached during PCR cycling, the polymerase dissociates from its inhibitor and commences polymerization. Use of hot start DNA polymerases is most often recommended for high-throughput applications, experiments requiring a high degree of specificity, or even routine PCR where the added security offered by a hot start enzyme is desired.

Choose Type:

FAQs for Hot Start PCR
Protocols for Hot Start PCR
Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please email us at busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.