- Protocol for OneTaq 2X Master Mix with GC Buffer (M0483)
- PCR Protocol Phusion® DNA Polymerase
- Protocol for Phusion® Hot Start Flex 2X Master Mix
- PCR Using NEBNext® High-Fidelity 2X PCR Master Mix (M0541)
- PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493)
- Protocol for Q5® Hot Start High-Fidelity 2X Master Mix
- Protocol for OneTaq® 2X Master Mix with Standard Buffer (M0482)
- PCR Protocol for Phusion® Hot Start Flex DNA Polymerase (M0535)
- Protocol Phusion® High-Fidelity PCR Master Mix with HF Buffer
- Protocol for OneTaq Hot Start 2X Master Mix with Standard Buffer (M0484)
- PCR Optimization with Phusion® High-Fidelity PCR Kit
- Protocol for a Routine PCR with Phusion® High-Fidelity PCR Kit
- PCR Optimization of the Control Template using Phusion® High-Fidelity PCR Kit
- Protocol for Phusion® High-Fidelity PCR Master Mix with GC Buffer
- Protocol for Q5® High-Fidelity 2X Master Mix
- PCR Optimization (E0555)
- Protocol for a Routine PCR (E0555)
- PCR Using Q5® High-Fidelity DNA Polymerase (M0491)
- Loop-mediated Isothermal Amplification (LAMP)
- Protocol for a PCR reaction using NEBNext® Q5® Hot Start HiFi PCR Master Mix (M0543)
Understanding Variability in DNA Amplification Reactions
Anatomy of a Polymerase - How Function and Structure are Related
Read about the relationship between Polymerase structure and function when copying DNA.
Polymerase Fidelity: What is it, and what does it mean for your PCR?
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- Taq PCR Kit Troubleshooting Guide
- Choosing RNA Input Amounts for NEB T2010
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
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Types of PCR
High-fidelity, multiplex, and Fast PCR are just a few of the types of amplification reactions you may need to conduct. NEB offers a variety of DNA polymerases for all of your experimental needs. Breton explains which polymerases to choose.