Helicase-dependent amplification (HDA) employs the double-stranded DNA unwinding activity of a helicase to separate strands, enabling primer annealing and extension by a strand-displacing DNA polymerase. This method was originally developed at NEB and is now used in a number of FDA-approved diagnostic assays. Like PCR, this system requires only two primers and produces short, discrete DNA products.
|Reaction Temperature||Amplicon Size||Detection Method(s)|
|65°C||<150 nt||Lateral flow|
- One-Step tHDA (thermostable HDA)
- One-Step RT-HDA (Reverse Transcription tHDA)
- One-Step qHDA (Real-time quantitative tHDA)
- One-Step qRT-HDA (Real-time quantitative RT-HDA)
- Two-Step tHDA (thermostable HDA)
- Two-Step RT-HDA (Reverse Transcription tHDA)
- Two-Step qHDA (Real-time quantitative tHDA)
- Two-Step qRT-HDA (Real-time quantitative RT-HDA)
- Protocol for unwinding double stranded DNA with Tte UvrD Helicase (#M1202)
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