Helicase-dependent Amplification
Helicase-dependent amplification (HDA) employs the double-stranded DNA unwinding activity of a helicase to separate strands, enabling primer annealing and extension by a strand-displacing DNA polymerase. This method was originally developed at NEB and is now used in a number of FDA-approved diagnostic assays. Like PCR, this system requires only two primers and produces short, discrete DNA products.
| Reaction Temperature | Amplicon Size | Detection Method(s) |
|---|---|---|
| 65°C | <150 nt | Lateral flow |
HDA utilizes a helicase to separate DNA in the presence of single-stranded binding proteins, enabling primer annealing and extension by a strand-displacing DNA polymerase.
Choose Type:
- One-Step tHDA (thermostable HDA)
- One-Step RT-HDA (Reverse Transcription tHDA)
- One-Step qHDA (Real-time quantitative tHDA)
- One-Step qRT-HDA (Real-time quantitative RT-HDA)
- Two-Step tHDA (thermostable HDA)
- Two-Step RT-HDA (Reverse Transcription tHDA)
- Two-Step qHDA (Real-time quantitative tHDA)
- Two-Step qRT-HDA (Real-time quantitative RT-HDA)
- Protocol for unwinding double stranded DNA with Tte UvrD Helicase (#M1202)
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While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
