
Site Directed Mutagenesis
Site-directed mutagenesis (SDM) is a method to create specific, targeted changes in double stranded plasmid DNA. There are many reasons to make specific DNA alterations (insertions, deletions and substitutions), including:
- To study changes in protein activity that occur as a result of the DNA manipulation.
- To select or screen for mutations (at the DNA, RNA or protein level) that have a desired property
- To introduce or remove restriction endonuclease sites or tags
Choose Type:
- 5 Minute Transformation Protocol using NEB 10-beta Competent E. coli (C3019H/C3019I)
- High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (High Efficiency) (C3019H/C3019I)
- Protocol for Q5® Hot Start High-Fidelity 2X Master Mix
- Optimizing Restriction Endonuclease Reactions
- Protocol for Control Reaction (E0554)
- Q5® Site-Directed Mutagenesis Kit Protocol (E0554)
- Q5® Site-Directed Mutagenesis Kit Quick Protocol (E0554)
- Protocol for Control Reaction (E0552)
- Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) Protocol (E0552)
- Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) Quick Protocol (E0552)
- Double Digest Protocol with Standard Restriction Enzymes
- KLD Enzyme Mix Reaction Protocol (M0554)
- Molecular Cloning Technical Guide
- Troubleshooting Guide for Cloning
Brochures
Troubleshooting Guides
- Yafeng Li, Delu Song, Ying Song, Liangliang Zhao, Natalie Wolkow, John W Tobias, Wenchao Song, Joshua L Dunaief 2015. Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)-β Signaling in the Retinal Pigment Epithelium. J Biol Chem. 290, PubMedID: 25802332, DOI: 10.1074/jbc.M115.645903
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