Cloning & Synthetic Biology



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Cloning & Synthetic Biology

Monarch® gDNA Binding Buffer

Monarch® gDNA Blood Lysis Buffer

Monarch® gDNA Cell Lysis Buffer

Monarch® gDNA Elution Buffer

Monarch® gDNA Purification Columns

Monarch® gDNA Tissue Lysis Buffer

Monarch® gDNA Wash Buffer

Monarch® Genomic DNA Purification Kit

Monarch® RNase A

Nuclease-free Water

PsiI-v2

Q5U®  Hot Start High-Fidelity DNA Polymerase

Thermolabile Proteinase K

DNA Analysis

1 kb DNA Ladder

1 kb Plus DNA Ladder

1 kb Plus DNA Ladder for Safe Stains

100 bp DNA Ladder

50 bp DNA Ladder

5-methyl-dCTP

Acyclonucleotide Set

AleI-v2

BsaI-HF®v2

BsiWI-HF®

BstZ17I-HF®

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

DNA Polymerase I, Large (Klenow) Fragment

Fast DNA Ladder

Hot Start Taq DNA Polymerase

Klenow Fragment (3'→5' exo-)

Lambda PFG Ladder

LongAmp® Taq DNA Polymerase

Low Molecular Weight DNA Ladder

MidRange PFG Marker

NEBNext® ChIP-seq Library Prep Master Mix Set for Illumina®

NEBNext® dA-Tailing Module

NEBNext® DNA Library Prep Master Mix Set for Illumina®

NEBNext® End Repair Module

NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® Fast DNA Library Prep Set for Ion Torrent™

pBR322 DNA-BstNI Digest

pBR322 DNA-MspI Digest

PCR Marker

PsiI-v2

Quick-Load® 1 kb DNA Ladder

Quick-Load® 1 kb Extend DNA Ladder

Quick-Load® 1 kb Plus DNA Ladder

Quick-Load® 100 bp DNA Ladder

Quick-Load® Purple 1 kb DNA Ladder

Quick-Load® Purple 1 kb Plus DNA Ladder

Quick-Load® Purple 100 bp DNA Ladder

Quick-Load® Purple 50 bp DNA Ladder

Quick-Load® Purple Low Molecular Weight DNA Ladder

Recombinant Albumin, Molecular Biology Grade

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

Supercoiled DNA Ladder

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with ThermoPol® Buffer

ThermoPol® Reaction Buffer Pack

ThermoPol® II (Mg-free) Reaction Buffer Pack

TriDye™ 1 kb DNA Ladder

TriDye™ 1 kb Plus DNA Ladder

TriDye™ 100 bp DNA Ladder

TriDye™ Ultra Low Range DNA Ladder

λ DNA-BstEII Digest

λ DNA-HindIII Digest

λ DNA-Mono Cut Mix

ΦX174 DNA-Hae III Digest

DNA Assembly and Cloning

BbsI

BbsI-HF®

BioBrick® Assembly Kit

BsaI

BsaI-HF®v2

BsmBI

EcoRI-HF®

Exonuclease V (RecBCD)

Gibson Assembly® Cloning Kit

Gibson Assembly® Master Mix

NEB® Golden Gate Assembly Kit (BsaI-HF®v2)

NEB® Golden Gate Assembly Mix

NEBuffer™ 2.1

NEBuilder® HiFi DNA Assembly Bundle for Large Fragments

NEBuilder® HiFi DNA Assembly Cloning Kit

NEBuilder® HiFi DNA Assembly Master Mix

Phusion® High-Fidelity DNA Polymerase

PstI

Q5® High-Fidelity DNA Polymerase

Quick Ligation™ Kit

SpeI

SpeI-HF®

T4 DNA Ligase

T5 Exonuclease

T7 DNA Ligase

Taq DNA Ligase

Thermolabile USER® II Enzyme

USER® Enzyme

XbaI

DNA End Modification

5-hydroxymethyluridine DNA Kinase

Antarctic Phosphatase

Antarctic Phosphatase Reaction Buffer

Deoxynucleotide (dNTP) Solution Set

DNA Polymerase I, Large (Klenow) Fragment

Klenow Fragment (3'→5' exo-)

Mung Bean Nuclease

NEBNext® dA-Tailing Module

NEBNext® dA-Tailing Reaction Buffer

Quick Blunting™ and Quick Ligation™ Kits

Quick Blunting™ Kit

Quick CIP

Shrimp Alkaline Phosphatase (rSAP)

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

T4 DNA Ligase Reaction Buffer

T4 DNA Polymerase

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (3' phosphatase minus)

T4 Polynucleotide Kinase Reaction Buffer

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with ThermoPol® Buffer

Terminal Transferase

ThermoPol® Reaction Buffer Pack

ThermoPol® II (Mg-free) Reaction Buffer Pack

DNA Isolation

Thermolabile Proteinase K

β-Agarase I

DNA Ligation

9°N™ DNA Ligase

Blunt/TA Ligase Master Mix

E. coli DNA Ligase

ElectroLigase®

HiFi Taq DNA Ligase

Instant Sticky-end Ligase Master Mix

NEBNext® Quick Ligation Module

NEBuffer Set (EcoRI, DpnII)

Quick Blunting™ and Quick Ligation™ Kits

Quick Ligation™ Kit

T3 DNA Ligase

T4 DNA Ligase

T4 DNA Ligase Reaction Buffer

T7 DNA Ligase

Taq DNA Ligase

Taq DNA Ligase Reaction Buffer

Thermostable 5´ App DNA/RNA Ligase

DNA Preparation

3´-Biotin-GTP

3´-Desthiobiotin-GTP

5-methyl-dCTP

7-deaza-dGTP

AatII

AbaSI

Acc65I

AccI

AciI

AclI

AcuI

Acyclonucleotide Set

Adenosine 5'-Triphosphate (ATP)

AfeI

AflII

AflIII

AgeI

AgeI-HF®

AhdI

AleI

AleI-v2

AluI

AlwI

AlwNI

AMV Reverse Transcriptase

ApaI

ApaLI

ApeKI

ApoI

ApoI-HF®

AscI

AseI

AsiSI

AvaI

AvaII

AvrII

BaeGI

BaeI

BamHI

BamHI-HF®

BanI

BanII

BbsI

BbsI-HF®

BbvCI

BbvI

BccI

BceAI

BcgI

BciVI

BclI

BclI-HF

BcoDI

BfaI

BfuAI

BglI

BglII

BlpI

BmgBI

BmrI

BmtI

BmtI-HF®

BpmI

Bpu10I

BpuEI

BSA, Molecular Biology Grade

BsaAI

BsaBI

BsaHI

BsaI

BsaI-HF®v2

BsaJI

BsaWI

BsaXI

BseRI

BseYI

BsgI

BsiEI

BsiHKAI

BsiWI

BslI

BsmAI

BsmBI

BsmFI

BsmI

BsoBI

Bsp1286I

BspCNI

BspDI

BspEI

BspHI

BspMI

BspQI

BsrBI

BsrDI

BsrFI

BsrFI-v2

BsrGI

BsrI

BssHII

BssKI

BssSI-v2

BstAPI

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstYI

BstZ17I

Bsu DNA Polymerase, Large Fragment

Bsu36I

BtgI

BtgZI

BtsCI

BtsIMutI

BtsI-v2

Cac8I

ClaI

CspCI

CutSmart® Buffer

CviAII

CviKI-1

CviQI

dATP Solution

DdeI

Deep Vent® (exo-) DNA Polymerase

Deep Vent® DNA Polymerase

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

Diluent A

Diluent B

Diluent C

DNA Polymerase I (E. coli)

DNA Polymerase I, Large (Klenow) Fragment

DpnI

DpnII

DraI

DraIII-HF®

DrdI

E. coli Poly(A) Polymerase

EaeI

EagI

EagI-HF®

EarI

EciI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

EpiMark® Hot Start Taq DNA Polymerase

FatI

FauI

Fnu4HI

FokI

FseI

FspEI

FspI

HaeII

HaeIII

Hemo KlenTaq®

HgaI

HhaI

HincII

HindIII

HindIII-HF®

HinfI

HinP1I

Hot Start Taq 2X Master Mix

Hot Start Taq DNA Polymerase

HpaI

HpaII

HphI

Hpy166II

Hpy188I

Hpy188III

Hpy99I

HpyCH4III

HpyCH4IV

HpyCH4V

I-CeuI

I-SceI

Isothermal Amplification Buffer II Pack

Isothermal Amplification Buffer Pack

KasI

Klenow Fragment (3'→5' exo-)

KpnI

KpnI-HF®

LongAmp® Hot Start Taq 2X Master Mix

LongAmp® Hot Start Taq DNA Polymerase

LongAmp® Taq 2X Master Mix

LongAmp® Taq DNA Polymerase

LongAmp® Taq PCR Kit

LpnPI

LunaScript RT SuperMix Kit

M13KO7 Helper Phage

Magnesium Chloride (MgCl2) Solution

Magnesium Sulfate (MgSO4) Solution

MboI

MboII

McrBC

MfeI

MfeI-HF®

MluCI

MluI

MluI-HF®

MlyI

MmeI

M-MuLV Reverse Transcriptase

MnlI

MscI

MseI

MslI

MspA1I

MspI

MspJI

Multiplex PCR 5X Master Mix

MwoI

NaeI

NarI

NciI

NcoI

NcoI-HF®

NdeI

NEBNext® High-Fidelity 2X PCR Master Mix

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

NEBNext® Ultra™ II Q5® Master Mix

NEBuffer™ 1

NEBuffer™ 1.1

NEBuffer™ 2

NEBuffer™ 2.1

NEBuffer™ 3

NEBuffer™ 3.1

NEBuffer™ 4

NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

NgoMIV

NheI

NheI-HF®

NlaIII

NlaIV

NmeAIII

NotI

NotI-HF®

NruI

NruI-HF®

NsiI

NsiI-HF®

NspI

Oligo d(T)18 mRNA Primer

Oligo d(T)23 VN

OneTaq® 2X Master Mix with Standard Buffer

OneTaq® DNA Polymerase

OneTaq® Hot Start 2X Master Mix with GC Buffer

OneTaq® Hot Start 2X Master Mix with Standard Buffer

OneTaq® Hot Start DNA Polymerase

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

OneTaq® One-Step RT-PCR Kit

OneTaq® Quick-Load® DNA Polymerase

OneTaq® RT-PCR Kit

OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

PacI

PaeR7I

PciI

PflFI

PflMI

Phusion® GC Buffer Pack

Phusion® HF Buffer Pack

Phusion® High-Fidelity DNA Polymerase

Phusion® High-Fidelity PCR Master Mix with GC Buffer

Phusion® High-Fidelity PCR Master Mix with HF Buffer

Phusion® Hot Start Flex 2X Master Mix

Phusion® Hot Start Flex DNA Polymerase

Phusion® High-Fidelity PCR Kit

PI-PspI

PI-SceI

PleI

PmeI

PmlI

Poly(U) Polymerase

PpuMI

PreCR® Repair Mix

ProtoScript® First Strand cDNA Synthesis Kit

ProtoScript® II First Strand cDNA Synthesis Kit

ProtoScript® II Reverse Transcriptase

PshAI

PsiI

PsiI-v2

PspGI

PspOMI

PspXI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

Q5® High-Fidelity 2X Master Mix

Q5® High-Fidelity DNA Polymerase

Q5® High-Fidelity PCR Kit

Q5® Hot Start High-Fidelity 2X Master Mix

Q5® Hot Start High-Fidelity DNA Polymerase

Q5® Reaction Buffer Pack

Quick Blunting™ Kit

Quick-Load® Taq 2X Master Mix

Random Primer 9

Recombinant Albumin, Molecular Biology Grade

Ribonucleotide Solution Mix

Ribonucleotide Solution Set

RNase H

RNase Inhibitor, Murine

RsaI

RsrII

SacI

SacI-HF®

SacII

S-adenosylmethionine (SAM)

SalI

SalI-HF®

SapI

Sau3AI

Sau96I

SbfI

SbfI-HF®

ScaI-HF®

ScrFI

SexAI

SfaNI

SfcI

SfiI

SfoI

SgrAI

SmaI

SmlI

SnaBI

SP6 RNA Polymerase

SpeI

SpeI-HF®

SphI

SphI-HF®

SrfI

SspI

SspI-HF®

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

StuI

StyD4I

StyI

StyI-HF®

Sulfolobus DNA Polymerase IV

SwaI

T3 RNA Polymerase

T4 DNA Polymerase

T4 RNA Ligase 2, truncated KQ

T7 DNA Polymerase (unmodified)

Taq 2X Master Mix

Taq 5X Master Mix

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with ThermoPol® Buffer

Taq PCR Kit

TaqI-v2

Terminal Transferase

TfiI

ThermoPol® Reaction Buffer Pack

ThermoPol® II (Mg-free) Reaction Buffer Pack

Thermostable RNase H

TliI

TseI

Tsp45I

Tsp509I

TspMI

TspRI

Tth111I

Vent® (exo-) DNA Polymerase

Vent® DNA Polymerase

WarmStart® RTx Reverse Transcriptase

XbaI

XcmI

XhoI

XmaI

XmnI

ZraI

β-Nicotinamide adenine dinucleotide (NAD+)

Fast Cloning: Accelerate your cloning workflows with reagents from NEB

AatII

Acc65I

AccI

AciI

AclI

AcuI

AflII

AgeI-HF®

AluI

AlwNI

ApaI

ApaLI

ApeKI

ApoI

ApoI-HF®

AscI

AseI

AvaI

AvaII

AvrII

BaeGI

BaeI

BamHI

BbsI

BbvI

BclI-HF

BcoDI

BfuAI

BglI

BglII

BmgBI

BmtI-HF®

BpmI

BpuEI

BsaHI

BsaI-HF®v2

BsaWI

BsaXI

BseRI

BsgI

BsiWI

BsiWI-HF®

BslI

BsmAI

BsmBI

BsmI

BsoBI

Bsp1286I

BspCNI

BspHI

BspQI

BsrBI

BsrDI

BsrGI

BsrI

BssHII

BstEII

BstNI

BstUI

BstXI

BstYI

BstZ17I-HF®

Bsu36I

BtgI

BtsCI

Cac8I

ClaI

CviAII

CviQI

DdeI

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EagI

EagI-HF®

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRV

EcoRV-HF®

Esp3I

Fnu4HI

FseI

HaeIII

HincII

HindIII-HF®

HinfI

HpaII

HphI

HpyAV

HpyCH4IV

HpyCH4V

Instant Sticky-end Ligase Master Mix

MluCI

MluI

MlyI

MnlI

MseI

MslI

MspA1I

MspI

NciI

NcoI

NcoI-HF®

NdeI

NEB® Turbo Competent E. coli (High Efficiency)

NgoMIV

NheI

NheI-HF®

NlaIII

NotI-HF®

NruI

NsiI

NspI

PacI

PaeR7I

PflMI

PshAI

PstI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

Q5® High-Fidelity 2X Master Mix

Q5® High-Fidelity DNA Polymerase

Q5® High-Fidelity PCR Kit

Q5® Hot Start High-Fidelity 2X Master Mix

Q5® Hot Start High-Fidelity DNA Polymerase

Q5® Reaction Buffer Pack

Quick Blunting™ and Quick Ligation™ Kits

Quick Blunting™ Kit

Quick CIP

Quick Ligation™ Kit

RsaI

SacI

SacI-HF®

SacII

SalI

SbfI

SbfI-HF®

ScaI-HF®

SfoI

SmaI

SpeI

SpeI-HF®

SphI-HF®

SspI-HF®

StuI

StyD4I

StyI

StyI-HF®

SwaI

TaqI-v2

TfiI

TseI

TspMI

TspRI

Tth111I

XbaI

XmaI

Nucleic Acid Purification

Monarch® DNA Cleanup Binding Buffer

Monarch® DNA Cleanup Columns (5 μg)

Monarch® DNA Elution Buffer

Monarch® DNA Gel Extraction Kit

Monarch® DNA Wash Buffer

Monarch® gDNA Binding Buffer

Monarch® gDNA Blood Lysis Buffer

Monarch® gDNA Cell Lysis Buffer

Monarch® gDNA Elution Buffer

Monarch® gDNA Purification Columns

Monarch® gDNA Tissue Lysis Buffer

Monarch® gDNA Wash Buffer

Monarch® Gel Dissolving Buffer

Monarch® Genomic DNA Purification Kit

Monarch® PCR & DNA Cleanup Kit (5 μg)

Monarch® Plasmid Lysis Buffer (B2)

Monarch® Plasmid Miniprep Columns

Monarch® Plasmid Miniprep Kit 

Monarch® Plasmid Resuspension Buffer (B1)

Monarch® Plasmid Wash Buffer 1

Monarch® Plasmid Wash Buffer 2

Monarch® RNase A

Monarch® Plasmid Neutralization Buffer (B3)

Site Directed Mutagenesis

DpnI

KLD Enzyme Mix

NEB® 10-beta Competent E. coli (High Efficiency)

Q5® Hot Start High-Fidelity 2X Master Mix

Q5® Site-Directed Mutagenesis Kit

Q5® Site-Directed Mutagenesis Kit (Without Competent Cells)

SOC Outgrowth Medium

Transformation

dam-/dcm- Competent E. coli

NEB Cloning Competent E. coli Sampler

NEB® 10-beta Competent E. coli (High Efficiency)

NEB® 10-beta Electrocompetent E. coli

NEB® 5-alpha Competent E. coli (High Efficiency)

NEB® 5-alpha Competent E. coli (Subcloning Efficiency)

NEB® 5-alpha Electrocompetent E. coli

NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

NEB® Stable Competent E. coli (High Efficiency)

NEB® Turbo Competent E. coli (High Efficiency)

NEB® Turbo Electrocompetent E. coli

SOC Outgrowth Medium

USER® Cloning

NEB® 10-beta Competent E. coli (High Efficiency)

NEB® 5-alpha Competent E. coli (High Efficiency)

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

Q5U®  Hot Start High-Fidelity DNA Polymerase

Thermolabile USER® II Enzyme

USER® Enzyme

DNA Analysis

1 kb DNA Ladder

1 kb Plus DNA Ladder

1 kb Plus DNA Ladder for Safe Stains

100 bp DNA Ladder

50 bp DNA Ladder

Fast DNA Ladder

Lambda PFG Ladder

Low Molecular Weight DNA Ladder

MidRange PFG Marker

pBR322 DNA-BstNI Digest

pBR322 DNA-MspI Digest

PCR Marker

PsiI-v2

Quick-Load® 1 kb DNA Ladder

Quick-Load® 1 kb Extend DNA Ladder

Quick-Load® 1 kb Plus DNA Ladder

Quick-Load® 100 bp DNA Ladder

Quick-Load® Purple 1 kb DNA Ladder

Quick-Load® Purple 1 kb Plus DNA Ladder

Quick-Load® Purple 100 bp DNA Ladder

Quick-Load® Purple 50 bp DNA Ladder

Quick-Load® Purple Low Molecular Weight DNA Ladder

Supercoiled DNA Ladder

TriDye™ 1 kb DNA Ladder

TriDye™ 1 kb Plus DNA Ladder

TriDye™ 100 bp DNA Ladder

TriDye™ Ultra Low Range DNA Ladder

λ DNA-BstEII Digest

λ DNA-HindIII Digest

λ DNA-Mono Cut Mix

ΦX174 DNA-Hae III Digest

Colony PCR

Hot Start Taq DNA Polymerase

LongAmp® Taq DNA Polymerase

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with ThermoPol® Buffer

ThermoPol® Reaction Buffer Pack

ThermoPol® II (Mg-free) Reaction Buffer Pack

DNA Sequencing

5-methyl-dCTP

Acyclonucleotide Set

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

DNA Polymerase I, Large (Klenow) Fragment

Klenow Fragment (3'→5' exo-)

NEBNext® ChIP-seq Library Prep Master Mix Set for Illumina®

NEBNext® dA-Tailing Module

NEBNext® DNA Library Prep Master Mix Set for Illumina®

NEBNext® End Repair Module

NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® Fast DNA Library Prep Set for Ion Torrent™

Restriction Enzyme Digestion

AleI-v2

BsaI-HF®v2

BsiWI-HF®

BstZ17I-HF®

PsiI-v2

Recombinant Albumin, Molecular Biology Grade

DNA Assembly and Cloning

NEB® Golden Gate Assembly Kit (BsaI-HF®v2)

NEB® Golden Gate Assembly Mix

Thermolabile USER® II Enzyme

USER® Enzyme

NEBuilder® HiFi DNA Assembly

NEBuilder® HiFi DNA Assembly Bundle for Large Fragments

NEBuilder® HiFi DNA Assembly Cloning Kit

NEBuilder® HiFi DNA Assembly Master Mix

Gibson Assembly®

Gibson Assembly® Cloning Kit

Gibson Assembly® Master Mix

Phusion® High-Fidelity DNA Polymerase

Q5® High-Fidelity DNA Polymerase

T5 Exonuclease

Taq DNA Ligase

BioBrick® Assembly

BioBrick® Assembly Kit

EcoRI-HF®

NEBuffer™ 2.1

PstI

SpeI

SpeI-HF®

T4 DNA Ligase

XbaI

Golden Gate Assembly

BbsI

BbsI-HF®

BsaI

BsaI-HF®v2

BsmBI

Exonuclease V (RecBCD)

NEB® Golden Gate Assembly Kit (BsaI-HF®v2)

NEB® Golden Gate Assembly Mix

Quick Ligation™ Kit

T4 DNA Ligase

T7 DNA Ligase

DNA End Modification

5-hydroxymethyluridine DNA Kinase

Dephosphorylation

Antarctic Phosphatase

Antarctic Phosphatase Reaction Buffer

Quick CIP

Shrimp Alkaline Phosphatase (rSAP)

Phosphorylation (Kinase)

5-hydroxymethyluridine DNA Kinase

Quick Blunting™ and Quick Ligation™ Kits

Quick Blunting™ Kit

T4 DNA Ligase Reaction Buffer

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (3' phosphatase minus)

T4 Polynucleotide Kinase Reaction Buffer

A-tailing

Deoxynucleotide (dNTP) Solution Set

Klenow Fragment (3'→5' exo-)

NEBNext® dA-Tailing Module

NEBNext® dA-Tailing Reaction Buffer

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with ThermoPol® Buffer

Terminal Transferase

ThermoPol® Reaction Buffer Pack

ThermoPol® II (Mg-free) Reaction Buffer Pack

Blunting

DNA Polymerase I, Large (Klenow) Fragment

Mung Bean Nuclease

Quick Blunting™ and Quick Ligation™ Kits

Quick Blunting™ Kit

T4 DNA Polymerase

DNA Isolation

Thermolabile Proteinase K

β-Agarase I

DNA Ligation

HiFi Taq DNA Ligase

Non-Cloning Ligation

E. coli DNA Ligase

HiFi Taq DNA Ligase

NEBNext® Quick Ligation Module

Thermostable 5´ App DNA/RNA Ligase

Cloning Ligation

9°N™ DNA Ligase

Blunt/TA Ligase Master Mix

ElectroLigase®

Instant Sticky-end Ligase Master Mix

NEBuffer Set (EcoRI, DpnII)

Quick Blunting™ and Quick Ligation™ Kits

Quick Ligation™ Kit

T3 DNA Ligase

T4 DNA Ligase

T4 DNA Ligase Reaction Buffer

T7 DNA Ligase

Taq DNA Ligase

Taq DNA Ligase Reaction Buffer

DNA Preparation

AleI-v2

PsiI-v2

Reverse Transcription (cDNA Synthesis)

AMV Reverse Transcriptase

LunaScript RT SuperMix Kit

M-MuLV Reverse Transcriptase

ProtoScript® First Strand cDNA Synthesis Kit

ProtoScript® II First Strand cDNA Synthesis Kit

ProtoScript® II Reverse Transcriptase

WarmStart® RTx Reverse Transcriptase

Restriction Enzyme Digestion

AatII

AbaSI

Acc65I

AccI

AciI

AclI

AcuI

AfeI

AflII

AflIII

AgeI

AgeI-HF®

AhdI

AleI

AleI-v2

AluI

AlwI

AlwNI

ApaI

ApaLI

ApeKI

ApoI

ApoI-HF®

AscI

AseI

AsiSI

AvaI

AvaII

AvrII

BaeGI

BaeI

BamHI

BamHI-HF®

BanI

BanII

BbsI

BbsI-HF®

BbvCI

BbvI

BccI

BceAI

BcgI

BciVI

BclI

BclI-HF

BcoDI

BfaI

BfuAI

BglI

BglII

BlpI

BmgBI

BmrI

BmtI

BmtI-HF®

BpmI

Bpu10I

BpuEI

BSA, Molecular Biology Grade

BsaAI

BsaBI

BsaHI

BsaI

BsaI-HF®v2

BsaJI

BsaWI

BsaXI

BseRI

BseYI

BsgI

BsiEI

BsiHKAI

BsiWI

BslI

BsmAI

BsmBI

BsmFI

BsmI

BsoBI

Bsp1286I

BspCNI

BspDI

BspEI

BspHI

BspMI

BspQI

BsrBI

BsrDI

BsrFI

BsrFI-v2

BsrGI

BsrI

BssHII

BssKI

BssSI-v2

BstAPI

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstYI

BstZ17I

Bsu36I

BtgI

BtgZI

BtsCI

BtsIMutI

BtsI-v2

Cac8I

ClaI

CspCI

CutSmart® Buffer

CviAII

CviKI-1

CviQI

DdeI

Diluent A

Diluent B

Diluent C

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EaeI

EagI

EagI-HF®

EarI

EciI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

FatI

FauI

Fnu4HI

FokI

FseI

FspEI

FspI

HaeII

HaeIII

HgaI

HhaI

HincII

HindIII

HindIII-HF®

HinfI

HinP1I

HpaI

HpaII

HphI

Hpy166II

Hpy188I

Hpy188III

Hpy99I

HpyCH4III

HpyCH4IV

HpyCH4V

I-CeuI

I-SceI

KasI

KpnI

KpnI-HF®

LpnPI

MboI

MboII

McrBC

MfeI

MfeI-HF®

MluCI

MluI

MluI-HF®

MlyI

MmeI

MnlI

MscI

MseI

MslI

MspA1I

MspI

MspJI

MwoI

NaeI

NarI

NciI

NcoI

NcoI-HF®

NdeI

NEBuffer™ 1

NEBuffer™ 1.1

NEBuffer™ 2

NEBuffer™ 2.1

NEBuffer™ 3

NEBuffer™ 3.1

NEBuffer™ 4

NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

NgoMIV

NheI

NheI-HF®

NlaIII

NlaIV

NmeAIII

NotI

NotI-HF®

NruI

NruI-HF®

NsiI

NsiI-HF®

NspI

PacI

PaeR7I

PciI

PflFI

PflMI

PI-PspI

PI-SceI

PleI

PmeI

PmlI

PpuMI

PshAI

PsiI

PspGI

PspOMI

PspXI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

Recombinant Albumin, Molecular Biology Grade

RsaI

RsrII

SacI

SacI-HF®

SacII

S-adenosylmethionine (SAM)

SalI

SalI-HF®

SapI

Sau3AI

Sau96I

SbfI

SbfI-HF®

ScaI-HF®

ScrFI

SexAI

SfaNI

SfcI

SfiI

SfoI

SgrAI

SmaI

SmlI

SnaBI

SpeI

SpeI-HF®

SphI

SphI-HF®

SrfI

SspI

SspI-HF®

StuI

StyD4I

StyI

StyI-HF®

SwaI

TaqI-v2

TfiI

TliI

TseI

Tsp45I

Tsp509I

TspMI

TspRI

Tth111I

XbaI

XcmI

XhoI

XmaI

XmnI

ZraI

PCR

3´-Biotin-GTP

3´-Desthiobiotin-GTP

5-methyl-dCTP

7-deaza-dGTP

Acyclonucleotide Set

Adenosine 5'-Triphosphate (ATP)

AMV Reverse Transcriptase

Bsu DNA Polymerase, Large Fragment

dATP Solution

Deep Vent® (exo-) DNA Polymerase

Deep Vent® DNA Polymerase

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

DNA Polymerase I (E. coli)

DNA Polymerase I, Large (Klenow) Fragment

E. coli Poly(A) Polymerase

EpiMark® Hot Start Taq DNA Polymerase

Hemo KlenTaq®

Hot Start Taq 2X Master Mix

Hot Start Taq DNA Polymerase

Isothermal Amplification Buffer II Pack

Isothermal Amplification Buffer Pack

Klenow Fragment (3'→5' exo-)

LongAmp® Hot Start Taq 2X Master Mix

LongAmp® Hot Start Taq DNA Polymerase

LongAmp® Taq 2X Master Mix

LongAmp® Taq DNA Polymerase

LongAmp® Taq PCR Kit

M13KO7 Helper Phage

Magnesium Chloride (MgCl2) Solution

Magnesium Sulfate (MgSO4) Solution

M-MuLV Reverse Transcriptase

Multiplex PCR 5X Master Mix

NEBNext® High-Fidelity 2X PCR Master Mix

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

NEBNext® Ultra™ II Q5® Master Mix

Oligo d(T)18 mRNA Primer

Oligo d(T)23 VN

OneTaq® 2X Master Mix with Standard Buffer

OneTaq® DNA Polymerase

OneTaq® Hot Start 2X Master Mix with GC Buffer

OneTaq® Hot Start 2X Master Mix with Standard Buffer

OneTaq® Hot Start DNA Polymerase

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

OneTaq® One-Step RT-PCR Kit

OneTaq® Quick-Load® DNA Polymerase

OneTaq® RT-PCR Kit

OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Phusion® GC Buffer Pack

Phusion® HF Buffer Pack

Phusion® High-Fidelity DNA Polymerase

Phusion® High-Fidelity PCR Master Mix with GC Buffer

Phusion® High-Fidelity PCR Master Mix with HF Buffer

Phusion® Hot Start Flex 2X Master Mix

Phusion® Hot Start Flex DNA Polymerase

Phusion® High-Fidelity PCR Kit

Poly(U) Polymerase

PreCR® Repair Mix

ProtoScript® First Strand cDNA Synthesis Kit

ProtoScript® II First Strand cDNA Synthesis Kit

ProtoScript® II Reverse Transcriptase

Q5® High-Fidelity 2X Master Mix

Q5® High-Fidelity DNA Polymerase

Q5® High-Fidelity PCR Kit

Q5® Hot Start High-Fidelity 2X Master Mix

Q5® Hot Start High-Fidelity DNA Polymerase

Q5® Reaction Buffer Pack

Quick Blunting™ Kit

Quick-Load® Taq 2X Master Mix

Random Primer 9

Ribonucleotide Solution Mix

Ribonucleotide Solution Set

RNase H

RNase Inhibitor, Murine

SP6 RNA Polymerase

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

Sulfolobus DNA Polymerase IV

T3 RNA Polymerase

T4 DNA Polymerase

T4 RNA Ligase 2, truncated KQ

T7 DNA Polymerase (unmodified)

Taq 2X Master Mix

Taq 5X Master Mix

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with ThermoPol® Buffer

Taq PCR Kit

Terminal Transferase

ThermoPol® Reaction Buffer Pack

ThermoPol® II (Mg-free) Reaction Buffer Pack

Thermostable RNase H

Vent® (exo-) DNA Polymerase

Vent® DNA Polymerase

WarmStart® RTx Reverse Transcriptase

β-Nicotinamide adenine dinucleotide (NAD+)

Fast Cloning: Accelerate your cloning workflows with reagents from NEB

AatII

Acc65I

AccI

AciI

AclI

AcuI

AflII

AgeI-HF®

AluI

AlwNI

ApaI

ApaLI

ApeKI

ApoI

ApoI-HF®

AscI

AseI

AvaI

AvaII

AvrII

BaeGI

BaeI

BamHI

BbsI

BbvI

BclI-HF

BcoDI

BfuAI

BglI

BglII

BmgBI

BmtI-HF®

BpmI

BpuEI

BsaHI

BsaI-HF®v2

BsaWI

BsaXI

BseRI

BsgI

BsiWI

BsiWI-HF®

BslI

BsmAI

BsmBI

BsmI

BsoBI

Bsp1286I

BspCNI

BspHI

BspQI

BsrBI

BsrDI

BsrGI

BsrI

BssHII

BstEII

BstNI

BstUI

BstXI

BstYI

BstZ17I-HF®

Bsu36I

BtgI

BtsCI

Cac8I

ClaI

CviAII

CviQI

DdeI

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EagI

EagI-HF®

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRV

EcoRV-HF®

Esp3I

Fnu4HI

FseI

HaeIII

HincII

HindIII-HF®

HinfI

HpaII

HphI

HpyAV

HpyCH4IV

HpyCH4V

Instant Sticky-end Ligase Master Mix

MluCI

MluI

MlyI

MnlI

MseI

MslI

MspA1I

MspI

NciI

NcoI

NcoI-HF®

NdeI

NEB® Turbo Competent E. coli (High Efficiency)

NgoMIV

NheI

NheI-HF®

NlaIII

NotI-HF®

NruI

NsiI

NspI

PacI

PaeR7I

PflMI

PshAI

PstI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

Q5® High-Fidelity 2X Master Mix

Q5® High-Fidelity DNA Polymerase

Q5® High-Fidelity PCR Kit

Q5® Hot Start High-Fidelity 2X Master Mix

Q5® Hot Start High-Fidelity DNA Polymerase

Q5® Reaction Buffer Pack

Quick Blunting™ and Quick Ligation™ Kits

Quick Blunting™ Kit

Quick CIP

Quick Ligation™ Kit

RsaI

SacI

SacI-HF®

SacII

SalI

SbfI

SbfI-HF®

ScaI-HF®

SfoI

SmaI

SpeI

SpeI-HF®

SphI-HF®

SspI-HF®

StuI

StyD4I

StyI

StyI-HF®

SwaI

TaqI-v2

TfiI

TseI

TspMI

TspRI

Tth111I

XbaI

XmaI

Nucleic Acid Purification

Monarch® DNA Cleanup Binding Buffer

Monarch® DNA Cleanup Columns (5 μg)

Monarch® DNA Elution Buffer

Monarch® DNA Gel Extraction Kit

Monarch® DNA Wash Buffer

Monarch® gDNA Binding Buffer

Monarch® gDNA Blood Lysis Buffer

Monarch® gDNA Cell Lysis Buffer

Monarch® gDNA Elution Buffer

Monarch® gDNA Purification Columns

Monarch® gDNA Tissue Lysis Buffer

Monarch® gDNA Wash Buffer

Monarch® Gel Dissolving Buffer

Monarch® Genomic DNA Purification Kit

Monarch® PCR & DNA Cleanup Kit (5 μg)

Monarch® Plasmid Lysis Buffer (B2)

Monarch® Plasmid Miniprep Columns

Monarch® Plasmid Miniprep Kit 

Monarch® Plasmid Resuspension Buffer (B1)

Monarch® Plasmid Wash Buffer 1

Monarch® Plasmid Wash Buffer 2

Monarch® RNase A

Monarch® Plasmid Neutralization Buffer (B3)

Site Directed Mutagenesis

DpnI

KLD Enzyme Mix

NEB® 10-beta Competent E. coli (High Efficiency)

Q5® Hot Start High-Fidelity 2X Master Mix

Q5® Site-Directed Mutagenesis Kit

Q5® Site-Directed Mutagenesis Kit (Without Competent Cells)

SOC Outgrowth Medium

Transformation

dam-/dcm- Competent E. coli

NEB Cloning Competent E. coli Sampler

NEB® 10-beta Competent E. coli (High Efficiency)

NEB® 10-beta Electrocompetent E. coli

NEB® 5-alpha Competent E. coli (High Efficiency)

NEB® 5-alpha Competent E. coli (Subcloning Efficiency)

NEB® 5-alpha Electrocompetent E. coli

NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

NEB® Stable Competent E. coli (High Efficiency)

NEB® Turbo Competent E. coli (High Efficiency)

NEB® Turbo Electrocompetent E. coli

SOC Outgrowth Medium

USER® Cloning

NEB® 10-beta Competent E. coli (High Efficiency)

NEB® 5-alpha Competent E. coli (High Efficiency)

Q5U®  Hot Start High-Fidelity DNA Polymerase

Thermolabile USER® II Enzyme

USER® Enzyme

Applications of USER® and Thermolabile USER II Enzymes

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

Q5U®  Hot Start High-Fidelity DNA Polymerase

Thermolabile USER® II Enzyme

USER® Enzyme