Restriction Enzyme Digestion Products

Restriction enzymes, first described in 1971, are bacterially derived enzymes that cleave DNA. Evolutionarily, restriction enzymes arose as a bacterial self-defense mechanism; the genomes of invading organisms would be degraded, leading to an inability to replicate. Type II restriction enzymes generally recognize an inverted repeat palindrome. This recognition site structure leads to a symmetrical cleavage of both DNA strands and results in either blunt- or sticky-ends of the digested DNA. Blunt ends are universally compatible with other blunt-ended DNA and possess a 5’ phosphate group to promote ligation. Sticky ends, on the other hand, are stretches of single-stranded DNA that is capable of self-ligation or ligation with a complementary region of DNA from another molecule or organism.

Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type. 


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Restriction Enzyme Digestion

AatII

AbaSI

Acc65I

AccI

AciI

AclI

AcuI

AfeI

AflII

AflIII

AgeI

AgeI-HF®

AhdI

AleI

AleI-v2

AluI

AlwI

AlwNI

ApaI

ApaLI

ApeKI

ApoI

ApoI-HF®

AscI

AseI

AsiSI

AvaI

AvaII

AvrII

BaeGI

BaeI

BamHI

BamHI-HF®

BanI

BanII

BbsI

BbsI-HF®

BbvCI

BbvI

BccI

BceAI

BcgI

BciVI

BclI

BclI-HF

BcoDI

BfaI

BfuAI

BglI

BglII

BlpI

BmgBI

BmrI

BmtI

BmtI-HF®

BpmI

Bpu10I

BpuEI

BSA, Molecular Biology Grade

BsaAI

BsaBI

BsaHI

BsaI

BsaI-HF®v2

BsaJI

BsaWI

BsaXI

BseRI

BseYI

BsgI

BsiEI

BsiHKAI

BsiWI

BslI

BsmAI

BsmBI

BsmFI

BsmI

BsoBI

Bsp1286I

BspCNI

BspDI

BspEI

BspHI

BspMI

BspQI

BsrBI

BsrDI

BsrFI

BsrFI-v2

BsrGI

BsrI

BssHII

BssKI

BssSI-v2

BstAPI

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstYI

BstZ17I

Bsu36I

BtgI

BtgZI

BtsCI

BtsIMutI

BtsI-v2

Cac8I

ClaI

CspCI

CutSmart® Buffer

CviAII

CviKI-1

CviQI

DdeI

Diluent B

Diluent C

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EaeI

EagI

EagI-HF®

EarI

EciI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

FatI

FauI

Fnu4HI

FokI

FseI

FspEI

FspI

HaeII

HaeIII

HgaI

HhaI

HincII

HindIII

HindIII-HF®

HinfI

HinP1I

HpaI

HpaII

HphI

Hpy166II

Hpy188I

Hpy188III

Hpy99I

HpyCH4III

HpyCH4IV

HpyCH4V

I-CeuI

I-SceI

KasI

KpnI

KpnI-HF®

LpnPI

MboI

MboII

McrBC

MfeI

MfeI-HF®

MluCI

MluI

MluI-HF®

MlyI

MmeI

MnlI

MscI

MseI

MslI

MspA1I

MspI

MspJI

MwoI

NaeI

NarI

NciI

NcoI

NcoI-HF®

NdeI

NEBuffer™ 1

NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

NgoMIV

NheI

NheI-HF®

NlaIII

NlaIV

NmeAIII

NotI

NotI-HF®

NruI

NruI-HF®

NsiI

NsiI-HF®

NspI

PacI

PaeR7I

PciI

PflFI

PflMI

PI-PspI

PI-SceI

PleI

PmeI

PmlI

PpuMI

PshAI

PsiI

PspGI

PspOMI

PspXI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

Recombinant Albumin, Molecular Biology Grade

RsaI

RsrII

SacI

SacI-HF®

SacII

SalI

SalI-HF®

SapI

Sau3AI

Sau96I

SbfI

SbfI-HF®

ScaI-HF®

ScrFI

SexAI

SfaNI

SfcI

SfiI

SfoI

SgrAI

SmaI

SmlI

SnaBI

SpeI

SpeI-HF®

SphI

SphI-HF®

SrfI

SspI

SspI-HF®

StuI

StyD4I

StyI

StyI-HF®

SwaI

TaqI-v2

TfiI

TliI

TseI

Tsp45I

Tsp509I

TspMI

TspRI

Tth111I

XbaI

XcmI

XhoI

XmaI

XmnI

ZraI