Restriction Enzyme Digestion Products

Restriction enzymes, first described in 1971, are bacterially derived enzymes that cleave DNA. Evolutionarily, restriction enzymes arose as a bacterial self-defense mechanism; the genomes of invading organisms would be degraded, leading to an inability to replicate. Type II restriction enzymes generally recognize an inverted repeat palindrome. This recognition site structure leads to a symmetrical cleavage of both DNA strands and results in either blunt- or sticky-ends of the digested DNA. Blunt ends are universally compatible with other blunt-ended DNA and possess a 5’ phosphate group to promote ligation. Sticky ends, on the other hand, are stretches of single-stranded DNA that is capable of self-ligation or ligation with a complementary region of DNA from another molecule or organism.

Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type. 


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Restriction Enzyme Digestion

AatII

AbaSI

Acc65I

AccI

AciI

AclI

AcuI

AfeI

AflII

AflIII

AgeI

AgeI-HF®

AhdI

AleI

AleI-v2

AluI

AlwI

AlwNI

ApaI

ApaLI

ApeKI

ApoI

ApoI-HF®

AscI

AseI

AsiSI

AvaI

AvaII

AvrII

BaeGI

BaeI

BamHI

BamHI-HF®

BanI

BanII

BbsI

BbsI-HF®

BbvCI

BbvI

BccI

BceAI

BcgI

BciVI

BclI

BclI-HF

BcoDI

BfaI

BfuAI

BglI

BglII

BlpI

BmgBI

BmrI

BmtI

BmtI-HF®

BpmI

Bpu10I

BpuEI

BSA, Molecular Biology Grade

BsaAI

BsaBI

BsaHI

BsaI

BsaI-HF®v2

BsaJI

BsaWI

BsaXI

BseRI

BseYI

BsgI

BsiEI

BsiHKAI

BsiWI

BslI

BsmAI

BsmBI

BsmFI

BsmI

BsoBI

Bsp1286I

BspCNI

BspDI

BspEI

BspHI

BspMI

BspQI

BsrBI

BsrDI

BsrFI

BsrFαI

BsrGI

BsrI

BssHII

BssKI

BssSαI

BstAPI

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstYI

BstZ17I

Bsu36I

BtgI

BtgZI

BtsCI

BtsIMutI

BtsαI

Cac8I

ClaI

CspCI

CutSmart® Buffer

CviAII

CviKI-1

CviQI

DdeI

Diluent A

Diluent B

Diluent C

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EaeI

EagI

EagI-HF®

EarI

EciI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

FatI

FauI

Fnu4HI

FokI

FseI

FspEI

FspI

HaeII

HaeIII

HgaI

HhaI

HincII

HindIII

HindIII-HF®

HinfI

HinP1I

HpaI

HpaII

HphI

Hpy166II

Hpy188I

Hpy188III

Hpy99I

HpyCH4III

HpyCH4IV

HpyCH4V

I-CeuI

I-SceI

KasI

KpnI

KpnI-HF®

LpnPI

MboI

MboII

McrBC

MfeI

MfeI-HF®

MluCI

MluI

MluI-HF®

MlyI

MmeI

MnlI

MscI

MseI

MslI

MspA1I

MspI

MspJI

MwoI

NaeI

NarI

NciI

NcoI

NcoI-HF®

NdeI

NEBuffer™ 1

NEBuffer™ 1.1

NEBuffer™ 2

NEBuffer™ 2.1

NEBuffer™ 3

NEBuffer™ 3.1

NEBuffer™ 4

NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

NgoMIV

NheI

NheI-HF®

NlaIII

NlaIV

NmeAIII

NotI

NotI-HF®

NruI

NruI-HF®

NsiI

NsiI-HF®

NspI

PacI

PaeR7I

PciI

PflFI

PflMI

PI-PspI

PI-SceI

PleI

PmeI

PmlI

PpuMI

PshAI

PsiI

PspGI

PspOMI

PspXI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

Recombinant Albumin, Molecular Biology Grade

RsaI

RsrII

SacI

SacI-HF®

SacII

S-adenosylmethionine (SAM)

SalI

SalI-HF®

SapI

Sau3AI

Sau96I

SbfI

SbfI-HF®

ScaI-HF®

ScrFI

SexAI

SfaNI

SfcI

SfiI

SfoI

SgrAI

SmaI

SmlI

SnaBI

SpeI

SpeI-HF®

SphI

SphI-HF®

SrfI

SspI

SspI-HF®

StuI

StyD4I

StyI

StyI-HF®

SwaI

TaqαI

TfiI

TliI

TseI

Tsp45I

Tsp509I

TspMI

TspRI

Tth111I

XbaI

XcmI

XhoI

XmaI

XmnI

ZraI