Home Applications Cloning & Synthetic Biology DNA Ligation Non-Cloning Ligation

Non-Cloning Ligation

The ability to ligate two or more pieces of DNA together enzymatically has found utility in many applications in the life sciences. Library preparation for Next Generation Sequencing (NGS) typically incorporates a ligation step to add bar-coded adapters to fragmented DNA, a critical step in this popular workflow. Many novel detection methodologies incorporate the ligation of DNA probes followed by a polymerase-based amplification step. For example, single nucleotide polymorphism (SNP) detection using the Ligase Chain Reaction (LCR) is a well-known application using a non-cloning thermostable ligase. Many other detection methods capitalizing on the ligation of DNA are being employed by basic and applied research groups, as well as many molecular diagnostics (MDx) companies.

Choose Type:

Protocols for Non-Cloning Ligation
Application Notes Non-Cloning Ligation
    Publications related to Non-Cloning Ligation
  1. Anton, B.P., Morgan, R.D., Ezraty, B., Manta, B., Barras, F., Berkmen, M. 2019. Complete genome sequence of Escherichia coli BE104, an MC4100 drivative lacking the methionine reductive pathway Microbiol Resour Announc. 8 (29), PubMedID: 31296691, DOI: 10.1128/MRA.00721-19
  2. Potapov, V., Ong, J.L., Kucera, R.B., Langhorst, B.W., Bilotti, K., Pryor, J.M., Cantor, E.J., Canton, B., Knight, T.F., Evans, T.C., Lohman, G.J.S. 2018. Comprehensive profiling of four base overhang ligation fidelity by T4 DNA ligase and application to DNA assembly ACS Synthetic Biology. 7 (11), PubMedID: 30335370, DOI: 10.1021/acssynbio.8b00333
Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

  1. DNA Ligase

    DNA Ligase Fidelity: When does it matter?

    High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA.

Featured Products