
DNA Ligation

Choose Type:
- Ligation Protocol with T4 DNA Ligase (M0202)
- Electroporation Protocol (C2989)
- NEBNext Quick Ligation Module Protocol (E6056)
- Removal of Single-Stranded Extension Protocol using Mung Bean Nuclease (M0250)
- Transformation Protocol (M0367)
- Transformation Protocol (M0370)
- Ligation Protocol for Cloning with Instant Sticky-end Ligase Master Mix (M0370)
- Ligation Protocol for Cloning with Blunt/TA Ligase Master Mix (M0367)
- Protocol for ssDNA/RNA Ligation (M0319)
- Optimizing Restriction Endonuclease Reactions
- Ligation Protocol with T3 DNA Ligase (M0317)
- Ligation Protocol with T7 DNA Ligase (M0318)
- E. coli DNA Ligase Protocol (M0205)
- Protocol for 9°N DNA Ligase (M0238)
- Protocol for Taq DNA Ligase (M0208)
- Please see manual (NEB #E7445) for protocols
- Protocol for the Quick Blunting Kit (E1201)
- HiFi Taq DNA Ligase (M0647) Protocol
- Molecular Cloning Technical Guide
- Properties of DNA and RNA Ligases
- Phage Display Troubleshooting Guide
- Troubleshooting Guide for Cloning
- Troubleshooting Guide for Ligases
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Anton, B.P., Morgan, R.D., Ezraty, B., Manta, B., Barras, F., Berkmen, M. (2019) Complete genome sequence of Escherichia coli BE104, an MC4100 drivative lacking the methionine reductive pathway Microbiol Resour Announc; 8 (29), e00721-19. PubMedID: 31296691, DOI: 10.1128/MRA.00721-19
- Potapov, V., Ong, J.L., Kucera, R.B., Langhorst, B.W., Bilotti, K., Pryor, J.M., Cantor, E.J., Canton, B., Knight, T.F., Evans, T.C., Lohman, G.J.S. (2018) Comprehensive profiling of four base overhang ligation fidelity by T4 DNA ligase and application to DNA assembly ACS Synth Biol; 7 (11), PubMedID: 30335370, DOI: 10.1021/acssynbio.8b00333
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