The non-overlapping substrate specificity of SNAP-tag® and CLIP-tag™ enables the orthogonal and complementary labeling of two proteins simultaneously in the same cell. Furthermore, SNAP-tag and CLIP-tag based pulse-chase experiments allow for visualization of different generations of proteins and analysis of protein turnover in living cells (1-3). Additionally, there are a numbers of companion products designed to enable a wide variety of other applications including: Biotin substrates, SDS-PAGE specific SNAP-tag and CLIP-Vista labels, non-fluorescent blocking agents, resin and magnetic beads for pull-down applications, building blocks for the generation of custom substrates, and a SNAP-tag specific purified protein and antibody.
- Jansen, L. et al (2007) J.Cell. Biol. 176, 795-805. PMID: 17339380
- Farr, G.A. (2009) J. Cell. Biol. 186, 269-282. PMID: 19620635
- Milenkovic, L. (2010) J. Cell. Biol. 187, 365-374. PMID: 19948480
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
CLIP-tag™ is a trademark of New England Biolabs, inc.
- Labeling Mammalian Cell Lysates (S9147)
- Labeling Proteins in vitro (S9147)
- Labeling SNAP-tag Purified Protein In Vitro (P9312)
- Protocol for Anti-SNAP-tag® Antibody (Polyclonal) (P9310)
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
- Use SNAP-Capture Magnetic Beads (S9145)
SNAP-tag® Technologies: Tools to Study Protein Function
Read about the NEB’s set of protein tools for the specific labeling (SNAP-, CLIP-, ACP- and MCP-tags) of fusion proteins.
- Cellular Imaging & Analysis Brochure
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- SNAP-tag® and CLIP-tag™ Substrate Selection Chart
- Labeling with SNAP-tag® Technology Troubleshooting Guide
- Simultaneous dual protein labeling inside live cells
- Protein localization and translocation
- Pulse-chase experiments
- Receptor internalization studies
- Selective cell surface labeling
- Protein pull-down assays
- Protein detection in SDS-PAGE
- Flow cytometry
- High throughput binding assays in microtiter plates
- Biosensor interaction experiments
- FRET-based binding assays
- Single molecule labeling
- Super-resolution microscopy
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.