LOCUS pRS414 4788 bp DNA circular 05-DEC-2007 DEFINITION Cloning vector pRS414, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pRS414 ORGANISM Cloning vector pRS414 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4788) AUTHORS Sikorski,R.S. and Hieter,P. TITLE A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae JOURNAL Genetics 122 (1), 19-27 (1989) PUBMED 2659436 REFERENCE 2 (bases 1 to 4788) AUTHORS Christianson,T.W., Sikorski,R.S., Dante,M., Shero,J.H. and Hieter,P. TITLE Multifunctional yeast high-copy-number shuttle vectors JOURNAL Gene 110 (1), 119-122 (1992) PUBMED 1544568 REFERENCE 3 (bases 1 to 4788) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (05-DEC-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank accession U03448. FEATURES Location/Qualifiers source 1..4788 /organism="Cloning vector pRS414" /mol_type="other DNA" gene 468..1142 /gene="TRP1" CDS 468..1142 /gene="TRP1" /codon_start=1 /product="phosphoribosylanthranilate isomerase" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK" rep_origin complement(1241..1696) /note="f1 origin of replication (+ -)" gene complement(1479..2057) /gene="lacZalpha" CDS complement(1479..2057) /gene="lacZalpha" /codon_start=1 /product="beta-galactosidase alpha fragment" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD" misc_feature 1894..2001 /gene="lacZalpha" /note="multiple cloning site (KpnI-SacI)" rep_origin complement(2455..3042) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point); rop gene has been disrupted" gene complement(3214..4074) /gene="bla" CDS complement(3214..4074) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide complement(4006..4074) /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein" misc_structure 4215..4333 /note="CEN6 (yeast chromosome VI centromere sequence)" rep_origin 4346..4719 /note="ARS209; histone H4 autonomously replicating sequence (ARS)" BASE COUNT 1307 a 1053 c 1153 g 1275 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga 1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac 1921 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg 1981 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt 2041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 2101 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact 2161 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 2221 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 2281 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 2341 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 2401 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 2461 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 2521 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 2581 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 2641 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 2701 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 2761 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 2821 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 2881 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 2941 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 3001 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 3061 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 3121 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 3181 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 3241 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 3301 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 3361 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 3421 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 3481 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 3541 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 3601 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 3661 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 3721 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 3781 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 3841 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 3901 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 3961 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 4021 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 4081 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 4141 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 4201 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa 4261 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt 4321 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt 4381 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg 4441 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc 4501 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt 4561 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat 4621 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt 4681 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta 4741 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc //