LOCUS pGPS4 3899 bp DNA circular 22-OCT-2007 DEFINITION Transposon donor vector pGPS4, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Transposon donor vector pGPS4 ORGANISM Transposon donor vector pGPS4 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3899) AUTHORS Biery,M.C., Stewart,F.J., Stellwagen,A.E., Raleigh,E.A. and Craig,N.L. TITLE A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis JOURNAL Nucleic Acids Res 28 (5), 1067-1077 (2000) PUBMED 10666445 REFERENCE 2 (bases 1 to 3899) AUTHORS Anton,B.P. and Raleigh,E.A. TITLE Transposon-mediated linker insertion scanning mutagenesis of the Escherichia coli McrA endonuclease JOURNAL J Bacteriol 186 (17), 5699-5707 (2004) PUBMED 15317774 REFERENCE 3 (bases 1 to 3899) AUTHORS Anton,B.P. TITLE Direct Submission JOURNAL Submitted (22-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..3899 /organism="Transposon donor vector pGPS4" /mol_type="other DNA" rep_origin complement(369..678) /note="R6K origin of replication (counter-clockwise)" gene complement(917..2107) /gene="tet" CDS complement(917..2107) /gene="tet" /note="tetR (confers resistance to tetracycline)" /codon_start=1 /product="tetracycline efflux protein, class C" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTTVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" misc_feature 2494..3876 /note="Transprimer-2 transposable element" misc_feature 2494..2692 /note="Tn7R (right end)" gene complement(2798..3457) /gene="cat" CDS complement(2798..3457) /gene="cat" /note="camR (confers resistance to chloramphenicol)" /codon_start=1 /product="chloramphenicol acetyltransferase" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" misc_feature 3710..3876 /note="Tn7L (left end)" BASE COUNT 1015 a 982 c 981 g 921 t ORIGIN 1 ggtaccctgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct 61 ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga 121 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga 181 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct 241 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt 301 cagcgccctg caccattatg ttccggatct atgtcgggtg cggagaaaga ggtaatgaaa 361 tggcagatcc ctggcttgtt gtccacaacc gttaaacctt aaaagcttta aaagccttat 421 atattctttt ttttcttata aaacttaaaa ccttagaggc tatttaagtt gctgatttat 481 attaatttta ttgttcaaac atgagagctt agtacgtgaa acatgagagc ttagtacgtt 541 agccatgaga gcttagtacg ttagccatga gggtttagtt cgttaaacat gagagcttag 601 tacgttaaac atgagagctt agtacgtgaa acatgagagc ttagtacgta ctatcaacag 661 gttgaactgc tgatcttcgg atctatgtcg ggtgcggaga aagaggtaat gaaatggcat 721 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac 781 gaagcgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc 841 acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg 901 tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg 961 ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg 1021 ccgaggcggc ataaatcgcc gtgacgatca gcggtccaat gatcgaagtt aggctggtaa 1081 gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca 1141 tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg 1201 ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc 1261 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt 1321 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc 1381 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt 1441 gcatgataaa gaagacagtc ataagtgcgg cgacagtagt catgccccgc gcccaccgga 1501 aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact 1561 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga 1621 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac 1681 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga 1741 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc 1801 gtccggcgta gaggatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg 1861 ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga 1921 gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac 1981 tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca 2041 agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag 2101 atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa 2161 gcttatcgat gataagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat 2221 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 2281 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 2341 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg 2401 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga 2461 acaggcagag ctcttactgt catgccatcc gtatgtttaa acacaataaa gtcttaaact 2521 gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac 2581 tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa 2641 actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggggtcgacg 2701 cggccgctaa ctataacggt cctaaggtag cgagtttggc gcgccaaacg atatcggatc 2761 caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat 2821 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat 2881 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca 2941 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga 3001 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat 3061 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga 3121 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg 3181 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga 3241 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt 3301 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat 3361 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata 3421 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa 3481 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg 3541 aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg 3601 tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtatgg 3661 atccgatatc atttaaatct agggataaca gggtaatact agtgtcgacc aaccagataa 3721 gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta 3781 cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc 3841 acccctccca gttcccaact ttattgtgtt taaacaccgt aagatgcttt tcagtgact //