LOCUS pGPS3 4293 bp DNA circular 22-OCT-2007 DEFINITION Cloning vector pGPS3, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pGPS3 ORGANISM Cloning vector pGPS3 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4293) AUTHORS Biery,M.C., Stewart,F.J., Stellwagen,A.E., Raleigh,E.A. and Craig,N.L. TITLE A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis JOURNAL Nucleic Acids Res 28 (5), 1067-1077 (2000) PUBMED 10666445 REFERENCE 2 (bases 1 to 4293) AUTHORS Anton,B.P. TITLE Direct Submission JOURNAL Submitted (22-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..4293 /organism="Cloning vector pGPS3" /mol_type="other DNA" rep_origin complement(433..1021) /note="pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(1192..2052) /gene="bla" CDS complement(1192..2052) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide complement(1984..2052) /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein" misc_feature 2572..4270 /note="Transprimer-1 transposable element" misc_feature 2572..2769 /note="Tn7R (right end)" gene complement(3124..3939) /gene="aph(3')-Ia" CDS complement(3124..3939) /gene="aph(3')-Ia" /note="kanR (confers resistance to kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn903" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" misc_feature 4104..4270 /note="Tn7L (left end)" BASE COUNT 1149 a 1059 c 974 g 1111 t ORIGIN 1 ggtaccctgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct 61 ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga 121 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga 181 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct 241 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt 301 cagcgccctg caccattatg ttccggatct atgtcgggtg cggagaaaga ggtaatgaaa 361 tggcagatcc aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 421 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 601 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 661 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 721 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 781 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 841 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 901 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 961 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 1021 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 1081 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 1141 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 1201 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 1261 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 1321 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 1381 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 1441 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 1501 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 1561 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 1621 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 1681 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 1741 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 1801 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 1861 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 1921 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 1981 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 2041 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 2101 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg atctatgtcg 2161 ggtgcggaga aagaggtaat gaaatggcat ccggatctgc atcgcaggat gctgctggct 2221 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt 2281 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg 2341 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat 2401 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac 2461 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa 2521 cgagctggac gcggatgaac aggcagagct cttactgtca tgccatccgt atgtgggcgg 2581 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta 2641 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt 2701 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag 2761 aggggcgtgg ggtcgacgcg gccgctaact ataacggtcc taaggtagcg agtttaaacg 2821 atatcggatc cggccgccgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc 2881 aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt 2941 tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt 3001 tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa 3061 gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc 3121 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc 3181 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt 3241 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca 3301 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac 3361 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg 3421 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga 3481 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat 3541 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg 3601 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc 3661 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca 3721 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag 3781 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc 3841 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg 3901 cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt 3961 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca 4021 tcagagattt tgagacacaa cgtggcttac taggatccga tatcatttaa atctagggat 4081 aacagggtaa tactagtgtc gaccaaccag ataagtgaaa tctagttcca aactattttg 4141 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac 4201 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg 4261 tccgcccaca ccgtaagatg cttttctgtg act //