LOCUS pGLuc-Basic 4920 bp DNA circular 19-OCT-2007 DEFINITION Cloning vector pGLuc-Basic, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pGLuc-Basic ORGANISM Cloning vector pGLuc-Basic other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4920) AUTHORS Tzertzinis,G. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..4920 /organism="Cloning vector pGLuc-Basic" /mol_type="other DNA" misc_feature 12..68 /note="multiple cloning site (BglII-BamHI)" gene 76..633 /gene="GLuc" CDS 76..633 /gene="GLuc" /codon_start=1 /product="Gaussia princeps luciferase" /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADR GKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKE SAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKW LPQRCATFASKIQGQVDKIKGAGGD" sig_peptide 76..126 /gene="GLuc" /note="secretory signal sequence" promoter 1238..1573 /note="SV40 promoter (clockwise)" gene 1625..2419 /gene="aph(3')-II" CDS 1625..2419 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" rep_origin complement(3165..3753) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(3924..4784) /gene="bla" CDS complement(3924..4784) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1128 a 1305 c 1288 g 1199 t ORIGIN 1 gacggatcgg gagatcttgg aattctgcag atatcctcga gcccaagctt ggtaccgagc 61 tcggatccag ccaccatggg agtcaaagtt ctgtttgccc tgatctgcat cgctgtggcc 121 gaggccaagc ccaccgagaa caacgaagac ttcaacatcg tggccgtggc cagcaacttc 181 gcgaccacgg atctcgatgc tgaccgcggg aagttgcccg gcaagaagct gccgctggag 241 gtgctcaaag agatggaagc caatgcccgg aaagctggct gcaccagggg ctgtctgatc 301 tgcctgtccc acatcaagtg cacgcccaag atgaagaagt tcatcccagg acgctgccac 361 acctacgaag gcgacaaaga gtccgcacag ggcggcatag gcgaggcgat cgtcgacatt 421 cctgagattc ctgggttcaa ggacttggag cccatggagc agttcatcgc acaggtcgat 481 ctgtgtgtgg actgcacaac tggctgcctc aaagggcttg ccaacgtgca gtgttctgac 541 ctgctcaaga agtggctgcc gcaacgctgt gcgacctttg ccagcaagat ccagggccag 601 gtggacaaga tcaagggggc cggtggtgac taagcggccg ctcgagcatg catctagaaa 661 taattcttac tgtcatgcca agtaagatgc ttttctgtgc tgcaatagca ggcatgctgg 721 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 781 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 901 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg 961 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 1021 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 1081 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 1141 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 1201 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 1261 tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 1321 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 1381 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 1441 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 1501 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 1561 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt 1621 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 1681 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 1741 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 1801 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 1861 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 1921 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 1981 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 2041 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 2101 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 2161 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 2221 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 2281 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 2341 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 2401 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc 2461 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 2521 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 2581 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 2641 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 2701 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca 2761 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 2821 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 2881 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 2941 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 3001 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 3061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 3121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 3181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 3241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 3301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa 3361 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 3421 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 3481 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 3541 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3601 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 3661 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 3721 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 3781 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 3841 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 3901 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 3961 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 4021 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 4081 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 4141 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 4201 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 4261 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 4321 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 4381 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 4441 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 4501 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 4561 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 4621 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 4681 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 4741 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 4801 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 4861 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc //