LOCUS pACP-tagm 5088 bp DNA circular 21-JAN-2010 DEFINITION Cloning vector pACPm, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pACP-tagm ORGANISM Cloning vector pACP-tagm other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5088) AUTHORS Brecht,A. and Muentner,K. TITLE Direct Submission JOURNAL Submitted (21-JAN-2010) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..5088 /organism="Cloning vector pACP-tagm" /mol_type="other DNA" promoter 1..602 /note="CMV immediate early promoter region" misc_feature 651..686 /note="multiple cloning site MCS1 (SacI-HindIII)" gene 690..953 /gene="ACPwt" CDS 690..953 /gene="ACPwt" /note="ACPwt sequence 690..923" /codon_start=1 /product="wild type acyl carrier protein (ACP) from Escherichia coli (gene acpP)" /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQAPAGIGAPGS" misc_feature 920..974 /note="multiple cloning site MCS2 (NarI-KpnI)" promoter complement(997..1014) /note="T7 promoter" polyA_signal 1027..1410 /note="SV40 polyA" gene 1575..2435 /gene="bla" CDS 1575..2435 /gene="bla" /note="ampR (confers resistance to ampicillin) (mutant A182V)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2990..3784 /gene="aph(3')-II" CDS 2990..3784 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin) (mutant R177S)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3785..4234 /note="HSV-TK polyA" rep_origin 4392..4980 /note="pUC19 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)" BASE COUNT 1256 a 1282 c 1281 g 1269 t ORIGIN 1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc 661 gcggtggcgg ccgcgatatc aagcttacca tgagcactat cgaagaacgc gttaagaaaa 721 ttatcggcga acagctgggc gttaagcagg aagaagttac caacaatgct tctttcgttg 781 aagacctggg cgcggattct cttgacaccg ttgagctggt aatggctctg gaagaagagt 841 ttgatactga gattccggac gaagaagctg agaaaatcac caccgttcag gctgccattg 901 attacatcaa cggccaccag gcgcctgcag gtataggcgc gccaggatcc tgactcgagg 961 gggggcccgg taccaggtaa gtgtacccaa ttcgccctat agtgagtcgt attacaattc 1021 actcgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggagat ccaattttta 1081 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tcacagtccc 1141 aaggctcatt tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc 1201 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa 1261 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa 1321 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 1381 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta ataagcagaa 1441 ggccatcctg acggatggtc tttttgcgtt tctacaaact cttttgttta tttttctaaa 1501 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 1561 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 1621 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 1681 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 1741 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 1801 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt 1861 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 1921 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 1981 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 2041 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 2101 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 2161 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 2221 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 2281 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 2341 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 2401 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 2461 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 2521 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagaca 2581 ttgaaaaagg aagaatcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg 2641 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 2701 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 2761 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 2821 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 2881 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 2941 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3001 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3061 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3121 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 3181 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 3241 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 3301 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 3361 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 3421 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag aacatcaggg 3481 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 3541 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 3601 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 3661 tacccgtgat attgctgaag aacttggcgg cgaatgggct gaccgcttcc tcgtgcttta 3721 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 3781 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 3841 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 3901 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg 3961 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 4021 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg 4081 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 4141 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 4201 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat 4261 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 4321 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 4381 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 4441 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 4501 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 4561 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 4621 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 4681 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 4741 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 4801 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 4861 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 4921 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 4981 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 5041 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcc //