LOCUS M13KO7 8669 bp DNA circular 19-OCT-2007 DEFINITION Cloning vector M13KO7, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector M13KO7 ORGANISM Cloning vector M13KO7 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8669) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT Sequenced 2002 by L. Panganaban. Contains numerous nucleotide discrepancies with M13mp18 and M13KE introducing possible missense mutations to M13 phage proteins. M13 origin of replication is interrupted by two segments from pACYC177 including the p15A-derived origin of replication and kanamycin resistance gene. FEATURES Location/Qualifiers source 1..8669 /organism="Cloning vector M13KO7" /mol_type="other DNA" gene join(8268..8669,1..831) /gene="II" CDS join(8268..8669,1..831) /gene="II" /codon_start=1 /product="pII" /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSA RTVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGH NVFGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVI DHLRNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKM TSYQKEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQG KDLIFDLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYF GFYRRLVNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINV DFSSQRPDWYNEPVLKIA" gene 496..831 /gene="X" CDS 496..831 /gene="X" /codon_start=1 /product="pX" /translation="MNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRLVNE GYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRPDW YNEPVLKIA" gene 843..1106 /gene="V" CDS 843..1106 /gene="V" /codon_start=1 /product="pV" /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVL VKITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK" gene 1108..1209 /gene="VII" CDS 1108..1209 /gene="VII" /codon_start=1 /product="pVII" /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR" gene 1206..1304 /gene="IX" CDS 1206..1304 /gene="IX" /codon_start=1 /product="pIX" /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS" gene 1301..1522 /gene="VIII" CDS 1301..1522 /gene="VIII" /codon_start=1 /product="pVIII" /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEY IGYAWAMVVVIVGATIGIKLFKKFTSKAS" gene 1579..2853 /gene="III" CDS 1579..2853 /gene="III" /exception="alternative start codon" /codon_start=1 /transl_table=11 /product="pIII" /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKT LDRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEG GGTKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFR NRQGALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFV CEYQGQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDF DYEKMANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGAT GDFAGSNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFSAGKPYEFSIDCDKI NLFRGVFAFLLYVATFMYVFSTFANILRNKES" gene 2856..3194 /gene="VI" CDS 2856..3194 /gene="VI" /codon_start=1 /product="pVI" /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFL ALIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQ KIVSYLDWDK" gene 3196..4242 /gene="I" CDS 3196..4242 /gene="I" /codon_start=1 /product="pI" /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLP QVGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDK ERQPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTL YSLITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGV YSYLTPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPE VKKVVSQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTV SIKKGNSNEIVKCN" gene 3916..4242 /gene="XI" /note="also called I*" CDS 3916..4242 /gene="XI" /note="also called I*" /codon_start=1 /product="pXI" /translation="MKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVVSQT YDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGNSN EIVKCN" gene 4220..5500 /gene="IV" CDS 4220..5500 /gene="IV" /codon_start=1 /product="pIV" /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGE SVIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSIPSIIQKYNPNNQDYI DELPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKS SNVLSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFA AGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRIL TLSGQKGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIV LDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFL SKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL" rep_origin 5488..5830 /note="M13 origin of replication (- +) (fragment)" rep_origin complement(6046..6591) /note="p15A origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(7141..7956) /gene="aph(3')-Ia" CDS complement(7141..7956) /gene="aph(3')-Ia" /note="kanR (confers resistance to kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn903" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 8093..8130 /note="M13 origin of replication (- +) (fragment)" BASE COUNT 2185 a 1867 c 1818 g 2799 t ORIGIN 1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat 61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact 121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta 181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca 241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg 301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag 361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt 421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca 481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct 541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt 601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt 661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg 721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt 781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca 841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt 901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg 961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc 1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc 1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat 1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt 1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta 1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct 1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga 1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta 1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa 1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt 1561 tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc 1621 tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca 1681 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt 1741 ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca 1801 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt 1861 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct 1921 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa 1981 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt 2041 cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact 2101 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg 2161 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag 2221 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat 2281 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt 2341 ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt 2401 gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat 2461 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt 2521 gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact 2581 ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct 2641 ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct 2701 tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta 2761 ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg 2821 tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt 2881 tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc 2941 ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg 3001 ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact 3061 ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc 3121 tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg 3181 attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg 3241 ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat 3301 cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt 3361 cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat 3421 tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat 3481 acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt 3541 aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg 3601 cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct 3661 tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat 3721 gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat 3781 actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat 3841 tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta 3901 aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt 3961 tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg 4021 gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct 4081 cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat 4141 agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc 4201 attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt 4261 tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt 4321 tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg 4381 tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc 4441 tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta 4501 taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga 4561 tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac 4621 ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa 4681 gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt 4741 tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc 4801 aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga 4861 tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg 4921 cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt 4981 agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg 5041 tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat 5101 tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg 5161 tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt 5221 tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat 5281 tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact 5341 cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa 5401 aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt 5461 atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg 5521 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 5581 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 5641 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 5701 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 5761 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 5821 ctatctcggg acggatcgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc 5881 agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg 5941 ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca 6001 ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg 6061 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg 6121 actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc 6181 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga 6241 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc 6301 agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg 6361 caaaagcacc actggcagca gccactggta attgatttag aggagttagt cttgaagtca 6421 tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag 6481 ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg 6541 gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat 6601 cttattaagg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 6661 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 6721 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 6781 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 6841 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 6901 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccgattc gagctcgccc 6961 cggggatcga ccagttggtg attttgaact tttgctttgc cacggaacgg tctgcgttgt 7021 cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc 7081 gccgtcccgt caagtcagcg taatgctctg ccagtgttac aaccaattaa ccaattctga 7141 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat 7201 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca 7261 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc 7321 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac 7381 tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca 7441 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg 7501 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga 7561 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata 7621 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc 7681 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt 7741 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa 7801 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac 7861 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg 7921 cctcgagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat 7981 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca 8041 gagattttga gacacaacgt ggctttcccc ccccccccct gcaggtctcg ggctattctt 8101 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 8161 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaata tttgcttata 8221 caatcttcct gtttttgggg cttttctgat tatcaaccgg ggtacatatg attgacatgc 8281 tagttttacg attaccgttc atcgattctc ttgtttgctc cagactctca ggcaatgacc 8341 tgatagcctt tgtagacctc tcaaaaatag ctaccctctc cggcatgaat ttatcagcta 8401 gaacggttga atatcatatt gatggtgatt tgactgtctc cggcctttct cacccttttg 8461 aatctttacc tacacattac tcaggcattg catttaaaat atatgagggt tctaaaaatt 8521 tttatccttg cgttgaaata aaggcttctc ccgcaaaagt attacagggt cataatgttt 8581 ttggtacaac cgatttagct ttatgctctg aggctttatt gcttaatttt gctaattctt 8641 tgccttgcct gtatgattta ttggatgtt //