Tech Note: Breaking through the limitations of Golden Gate Assembly
Learn how ligase fidelity data and BsaI-HFv2 provide 20+ fragment Golden Gate assemblies that exhibit high efficiencies, >90% accuracy and low backgrounds.
NEB TV Ep. 22 - Technical Support at NEB
Go behind the scenes into NEB’s technical support program. Hear from our scientists as they describe memorable tech support moments and interesting collaborations that have resulted from tech support calls.
Behind the paper: NicE-seq high resolution open chromatin profiling
Behind the paper: Sriharsa and Chaithanya summarize their recent Genome Biology publication describing the NicE-seq method for open chromatin profiling and the advantages it offers over current chromatin accessibility profiling techniques.
Introducing the NEB Podcast Series
The NEB podcast series, Lessons from Lab & Life, is dedicated to sharing helpful life lessons from successful scientists. From an overview of cutting edge research to finding sources of inspiration, our podcasts can be enjoyed by science enthusiasts that like to ponder “what it all means”.
NEB TV Ep. 21 - RNA modifications
In this episode we are talking about the importance of RNA modifications. Hear about protocols for prokaryotic transcription start site (TSS) determination, as well as detection and quantitation of RNA modifications, and learn how the RNA Modification Database (http://mods.rna.albany.edu) can help your RNA research.
Learn Molecular Biology in Just 2 Weeks!
The New England Biolabs Molecular Biology Summer Workshop provides lecture-based learning and hands-on experience in DNA cloning, gene expression, PCR, genomics and bioinformatics, DNA sequencing, CRISPR/Cas9 gene editing, and much more.
Detailed Characterization of Antibody Glycan Structure Using the N-Glycan Sequencing Kit
Characterization of glycans on therapeutic IgGs is critical, but the inherent complexity of protein glycosylation presents a challenge. This paper describes how the N-Glycan Sequencing Kit can be used to precisely characterize glycans on the Fc domain of therapeutic antibodies.
New online tool: PCR Fidelity Estimator
Quickly estimate the percentage of correct DNA copies per cycle of PCR for selected DNA polymerases using our new online tool.
Probe-based qPCR: probe compatibility and multiplexing with Luna® Universal Probe qPCR Master Mix
Probe-based qPCR is a powerful diagnostic and quantitative tool, and is compatible with multiplexing. This application note highlights the compatibility of the Luna Universal Probe qPCR Master Mix with a variety of probe types, and demonstrates its use for detection of multiple targets in a single reaction.
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