McrA, McrBC and EcoKI Strain Phenotypes

  Mcr Mcr EcoK Strain Background
Strain A BC R M
1100 + + + +  
AB266 + + + +  
AB1157 + + + + Mrr+
AT2459 - + + +  
BNN93 - - - + C600
BNN102 - - - + C600
CH731* + ? ? ?  
CH1332* - ? ? ? CH731
CH1372* - ? ? ? CH731
C235 - + + +  
C600 *** + + + Y70; Mrr+
CES200 - + - + AB1157
CJ236**
- + + +  
CPB1293 - + + + W3110
CPB1321 - - - + W3110
CR63 - + + +  
CSR603 ? + + +  
c2813 - - - + WA802; Mrr+
DB1316* + - - + Mrr+
DH1 + + - + MM294; Mrr+
DH3 - + - + MM294; Mrr+
DH5 + + - + MM294; Mrr+
DH5aMCR* - Δ Δ Δ DH5; Mrr -
DH10B* - Δ Δ Δ MC1061; Mrr -
DL538* - - - + NM621
DS410* - + + +  
DM800 - + + +  
ED8641 - + - + WA803
ED8654 - + - + WA803
ED8739 - - - - WA803
ED8767 - - - - WA803
ER1370 + + + + NK7254 (JC1552)
ER1381 + + - + ER1370; Mrr+
ER1378 + - - + ER1370; Mrr+
ER1398 + - - + MM294; Mrr+
ER1414 + - - + W3110; Mrr+
ER1458 - - - + Y1084
ER1562 - - - + MM294; Mrr+
ER1563 - + - + MM294; Mrr+
ER1564 - + - + ER1370; Mrr+
ER1565 - - - + ER1370; Mrr+
ER1647* - Δ Δ Δ ER1370; Mrr -
ER1648 - Δ Δ Δ ER1370; Mrr -
ER1793* - Δ Δ Δ ER1370; Mrr -
ER1821** - Δ Δ Δ MM294; Mrr -
ER2267** - Δ Δ Δ MM294; F+; Mrr -
ER2925 - - - + GM271; Mrr +
FS1585 - + + + C600
GM31* - +/- + + Mrr+/-
GM161 ? + + +  
GM271 - - - + Mrr+
GM272 ? + + +  
GM2159** + + + + Mrr+
GM2929** - - - + GM2163 recF
GW1002 - ? + +  
H680 - ? + +  
HB101 + Δ Δ Δ Mrr -
Hfr3000 YA149 - + + + HfrH
Hfr3000 - + + +  
Hfr Cavalli + - + +  
Hfr Hayes** + + + + Mrr+
HfrH thi - - + + +  
HfrP4X6 + + + +  
HfrR4** + + + + Mrr+
HL1228* - - - + NM621
JC7623** - + + + Mrr+
JH132 - Δ Δ Δ K802
JM83 (+) + + +  
JM101 + (+) + + JM83
JM107 - + - + MM294; Mrr+
JM107MA2 - - - + JM107
JM109 (-) (+) - + JM107
5K - + - + C600
K12 + + + +  
K802 see WA802  
K803 see WA803  
K10 + - + + Hfr Cavalli
KMBL1164 - ? + +  
KW251* - - - +  
LE392 - + - + ED8654
MB406* - + - +  
MC1040 (+) (+)  
MC1061 - - - + Mrr+
MM294 + + - + 1100; Mrr+
NM477 - - - - C600
NM494 - + - - pop101
NM514 - &/or + - + pop101
NM519 + + - +  
NM538 (-) (+) - + ED8654
NM539 - + - + NM538
NM554 - - - + MC1061
NM621 - - - + C600
NM646* - - - + WA802
NM664* - Δ Δ Δ WA802; Mrr -
NM670* - + + + WA802
NM673* - - - + WA802
NM674* - Δ Δ Δ WA802; Mrr -
NW1* - - - + AT2459
NW2* - Δ Δ Δ AT2459; Mrr -
P678** + ? ? +  
PA309 + + + +  
PC0543** + - + + Mrr+
PC0950 + - + + Mrr+
Q358 - + - +  
Q359 - + - +  
RL88 - - + +  
RR1 + Δ Δ Δ HB101; Mrr -
SK5022 - + + +  
TC410* - Δ Δ Δ DS410; Mrr -
TC600* - - - + C600B
W6 + + + +  
W677** + ? + +  
W3110 + + + + Mrr+
W4797 + + + +  
WA704 - - + + C600 X HfrCavalli
WA802 - - - + WA704; Mrr+
WA803 - - - - WA704; Mrr+
WW3352 - + + +  
X149 - + + +  
Y10 + + + +  
Y53 + + + + Y10
Y70 + + + + Y53
Y1084 - + + + BNN96; SG1041
Y1088 - + - + LE392; ED8654
Y1090 (-) (+) + + Y1084
Non-K12 strains
E. coli B + + + +  
E. coli B/r ? + + +  
BL21** + + - - restricts l.Sss I
E. coli C - (-) - -  

Mcr phenotypes reported in [Raleigh, E.A. et al. (1988) Nucl. Acids Res., 16, 1563-1575 PMID: 2831502, References 7 in Restriction of Foreign DNA by E. coli K-12] except those denoted with asterisks. Mrr phenotypes determined at NEB except DH10B (11). EcoKI phenotypes sometimes inferred from genotype.

Table Notes:

  • ( ) phenotype inferred from that of ancestral or descendant strains.
  • ? phenotype not tested.
  • Δ mutation present is known to be a large deletion.
  • ‡ These strains were formerly thought to carry the mcrB allele present in E.coli B. They are now known to carry an extensive deletion of mrr -hsd -mcrBC (ref. 6; M. Noyer-Weidner, personal communication; J. Kelleher, personal communication).
  • * Phenotypes reported in one of references 8-12.
  • ** Phenotypes determined at New England Biolabs, Inc.
  • *** C600 lines obtained from different sources give different results. The original strain, and that obtained from the E. coli Genetic Stock Center (Yale University) are McrA+; derivatives traceable to the Brenner laboratory are McrA- (21).