Restriction Enzymes for Epigenetics Selection Chart

Several of NEB's restriction enzymes are EpiMark® validated can be used to study epigenetic modifications of DNA.

Product NEB# Size Application
MspJI R0661S/L 200/1,000 units ● Identify 5-hmC and 5-mC
LpnPI R0663S/L 200/1,000 units ● Identify 5-hmC and 5-mC
FspEI R0662S/L 200/1,000 units ● Identify 5-hmC and 5-mC
DpnI R0176S/L 1,000/5,000 units ● Identify 5-mA used with DpnII
DpnII R0543S/L 1,000/5,000 units ● Identify 5-mA used with DpnI
McrBC M0272S/L 500/2,500 units ● Identify methylation 
MspI R0106S/L 5,000/25,000 units ● Identify 5-mC used with HpaII
HpaII R0171S/L 2,000/10,000 units ● Identify 5-mC used with MspI

EpiMark® is a registered trademark of New England Biolabs, Inc.