Components | T4 DNA Ligase Reaction Buffer | B0202 | StickTogether™ DNA Ligase Buffer | B0535 | ThermoPol® Reaction Buffer | B9004 | T4 Polynucleotide Kinase Reaction Buffer | B0201 | NEBuffer™ EcoRI/SspI | B0101 | Antarctic Phosphatase Reaction Buffer | B0289 | Exonuclease I Reaction Buffer | B0293 | Exonuclease VII Reaction Buffer | B0379 | NEBuffer™ r1.1 | B6001 | Diluent A (with rAlbumin) | B8532 | Diluent B (with rAlbumin) | B8533 | Diluent C (with rAlbumin) | B8534 | NEBuffer™ r2.1 | B6002 | rCutSmart™ Buffer | B6004 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Tris-HCl | 50 mM | 66 mM | 20 mM | 70 mM | 100 mM | 50 mM | 10 mM | 10 mM | 10 mM | 10 mM | ||||
Bis-Tris-Propane-HCl | 50 mM | 10 mM | ||||||||||||
Tris-acetate | 20 mM | |||||||||||||
Sodium Phosphate | 50 mM | |||||||||||||
Potassium Acetate | 50 mM | |||||||||||||
Magnesium Acetate | 10 mM | |||||||||||||
EDTA | 8 mM | 0.1 mM | 0.1 mM | 0.1 mM | ||||||||||
NaCl | 50 mM | 300 mM | 250 mM | 50 mM | ||||||||||
KCl | 10 mM | 50 | ||||||||||||
MgCl2 | 10 mM | 10 mM | 10 mM | 10 mM | 1 mM | 6.7 mM | 10 mM | 10 mM | ||||||
ZnCl2 | 0.1 mM | |||||||||||||
MgSO4 | 2 mM | |||||||||||||
(NH4)2SO4 | 10 mM | |||||||||||||
Glycine-KOH | 67 mM | |||||||||||||
BSA | ||||||||||||||
Recombinant Albumin | 100 µg/ml | 200 µg/ml | 500 µg/ml | 200 µg/ml | 100 µg/ml | 100 µg/ml | ||||||||
ATP | 1 mM | 1 mM | ||||||||||||
DTT | 10 mM | 1 mM | 5 mM | 1 mM | 1 mM | 1 mM | ||||||||
β-ME | 10 mM | |||||||||||||
2-mercapto-ethanol | ||||||||||||||
Polyethylene glycol (PEG 6000) | 7.5% | |||||||||||||
Triton® X-100 | 0.1% | 0.025% | 0.15% | |||||||||||
Glycerol | 50% | 50% | 50% | |||||||||||
pH @ 25 | 7.50 | 7.60 | 8.80 | 7.60 | 7.50 | 6.00 | 9.50 | 8.00 | 7.00 | 7.40 | 7.40 | 7.40 | 7.90 | 7.90 |
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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