Double Digest Finder

Use this tool to guide your reaction buffer selection when setting up double digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product. Learn more information about double digests, including how to set up a reaction.

We also encourage you to use our online tool, NEBcloner, which provides you with a detailed double-digest protocol based upon the 2 restriction enzymes that you select.
  1. Double Digestion with NEBcloner

    Find out how easy it is to get double digest recommendations with NEBcloner.