Where can I find guidelines and protocols for using NEBNext RNA Depletion Core Reagent Set (NEB #E7865, NEB #E7870), including for RNA that is intact, partially degraded or degraded, and with NEBNext Ultra II Directional RNA and NEBNext Ultra II RNA reagents?

Click here for important guidelines on use of Custom RNA Depletion Probe Pools with the NEBNext RNA Depletion Core Reagent Set.
 
Several protocols for use of NEB #E7865 and #E7870 can be found within the product manual. Please refer to the following details to identify which protocol is required for your application:

  • Use of the NEBNext RNA Depletion Core Reagent Set (NEB #E7865, #E7870) with no library construction protocols
  • Use with Intact or Partially Degraded RNA and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760 or NEB #E7165)
  • Use with Intact or Partially Degraded RNA and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #7770 or NEB #E7775)
  • Use with Degraded RNA and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760 or NEB #E7165)
  • Use with Degraded RNA and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #7770 or NEB #E7775)