DNA Polymerase Selection Chart
Several factors govern which polymerase should be used in a given application, including:
Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template?
Removal of existing nucleotides:
Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end?
Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable?
Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?