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Nucleotide Solutions

New England Biolabs supplies a selection of highly pure nucleotides for use with DNA and RNA polymerases.

Deoxynucleotide Solution Set (NEB #N0446) 

The Deoxynucleotide Solution Set contains four separate 100 mM solutions of ultrapure nucleotides (dATP, dCTP, dGTP, and dTTP).

Deoxynucleotide Solution Mix (NEB #N0447)

The Deoxynucleotide Solution Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP. Each nucleotide is present at a concentration of 10 mM in the mixture for a total dNTP concentration of 40 mM.

7-deaza-dGTP* (NEB #N0445)

7-deaza-dGTP is a useful additive for the PCR of GC-rich templates; contains a 5 mM solution of 7-deaza-dGTP as a dilithium salt.
* licensed from Roche Diagnostics GmbH

Acyclonucleotide Set (NEB #N0460)

The Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP), supplied as a dry powder.

dATP Solution (NEB #N0440)

The dATP solution contains 0.25 ml of 100 mM ultrapure dATP.

Ribonucleotide Solution Set (NEB #N0450)

Ribonucleotide Solution Set consists of four separate 100 mM solutions of ATP, GTP, CTP and UTP.

Ribonucleotide Solution Mix (NEB #N0466)

The Ribonucleotide Solution Mix is an equimolar mixture of ribonucleotide triphosphates (rATP, rCTP, rGTP and rUTP). Each is supplied at a concentration of 25 mM for a total concentration of 100 mM.

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FAQs for Nucleotide Solutions
Protocols for Nucleotide Solutions
DNA Polymerase Selection Chart
NEB offers a guidelines for choosing the correct DNA polymerase for your application by providing a list of specific properites.
Several factors govern which polymerase should be used in a given application, including: 

Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template? 

Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end? 

Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable? 

Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?

PCR Selection Tool
Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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