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Product Information
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3.
Highlights
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Recognizes non-perfectly matched DNA
Product Source
An E. coli strain that carries a fusion of maltose binding protein and T7 Endonuclease I (T7 Endo I).- This product is related to the following categories:
- Mutation Detection Reagents and Kits Products,
- Genome Editing Products,
- Exonucleases and Non-specific Endonucleases Products, DNA Repair Enzymes and Structure-specific Endonucleases Products
- This product can be used in the following applications:
- Whole Genome Amplification & Multiple Displacement Amplification,
- Genome Editing Applications
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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Citations & Technical Literature
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Product Citation Tool
Additional Citations
- Zheng Hu, Wencheng Ding, Da Zhu, Lan Yu, Xiaohui Jiang, Xiaoli Wang, Changlin Zhang, Liming Wang, Teng Ji, Dan Liu, Dan He, Xi Xia, Tao Zhu, Juncheng Wei, Peng Wu, Changyu Wang, Ling Xi, Qinglei Gao, Gang Chen, Rong Liu, Kezhen Li, Shuang Li, Shixuan Wang, Jianfeng Zhou, Ding Ma, Hui Wang (2015) TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. J Clin Invest; 125, 425-36. PubMedID: 25500889, DOI: 10.1172/JCI78206
- A-Bk Dad, S Ramakrishna, M Song, H Kim (2014) Enhanced gene disruption by programmable nucleases delivered by a minicircle vector. Gene Ther; , PubMedID: 25142139, DOI: 10.1038/gt.2014.76
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