Glycobiology

Glycobiology

New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are not hydrolyzed by some exoglycosidases, we use only fluorescently-labeled oligosaccharides to screen for contaminating glycosidases.

To find all of our Glycobiology Application Notes, click here.

Glycobiology includes these subcategories:

Endoglycosidases
Exoglycosidases
Heparinases

    Publications related to Glycobiology:

  1. Magnelli P, Bielik A, Guthrie E.  (2012). Identification and Characterization of Protein Methods Mol Biol. . 801, 189-211.
  2. Magnelli PE, Bielik AM, Guthrie EP. (2011). Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases J Vis Exp. . 58, pii: 3749. DOI: 10.3791/3749
  3. Piro, J.R., Harris, B.T., Nishina, K., Soto, C., Morales, R., Rees, J.R., Supattapone, S. (2009). Prion protein glycosylation is not required for strain-specific neurotropism  J Virol.  . 83, 5321-8.
  4. Velho, A.M., Jarvis, S.M. (2009). Topological studies of hSVCT1, the human sodium-dependent vitamin C transporter and the influence of N-glycosylation on its intracellular targeting Exp. Cell Res. . 315, 2312-21.
  5. Boeggeman, E., Ramakrishnan, B., Pasek, M., Manzoni, M., Puri, A., Loomis, K.H., Waybright, T.J., Qasba, P.K. (2009). Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: application for cell surface antigen detection Bioconjug. Chem. . 20, 1228-36.
  6. Gong, B., Cukan, M., Fisher, R., Li, H., Stadheim, T.A., Gerngross, T. (2009). Characterization of N-linked glycosylation on recombinant glycoproteins produced in Pichia pastoris using ESI-MS and MALDI-TOF Methods Mol. Biol. . 534, 213-23.
  7. Rasmussen, T.N., Plenge, P., Bay, T., Egebjerg, J., Gether, U. (2009). A single nucleotide polymorphism in the human serotonin transporter introduces a new site for N-linked glycosylation Neuropharmacology . 57, 287-94.
  8. Lee, K.J., Jung, J.H., Lee, J.M,, So, Y., Kwon, O., Callewaert, N., Kang, H.A., Ko, K., Oh, D.B. (2009). High-throughput quantitative analysis of plant N-glycan using a DNA sequencer Biochem. Biophys. Res. Commun.  . 380, 223-9.
  9. Lee, K.J., Jung, J.H., Lee, J.M., So, Y., Kwon, O., Callewaert, N., Kang, H.A., Ko, K., Oh, D.B. (2009). High-throughput quantitative analysis of plant N-glycan using a DNA sequencer Biochem. Biophys. Res. Commun. . 380, 223-9.
  10. Gefter, J.V., Shaufl, A.L., Fink, M.P., Delude, R.L. (2009). Comparison of distinct protein isoforms of the receptor for advanced glycation end-products expressed in murine tissues and cell lines Cell Tissue Res. . 337, 79-89.
  11. Zhuo, Y., Chammas, R., Bellis, S.L. (2008). Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis J. Biol. Chem. . 283, 22177-85.
  12. Fujita, N., Tamura, A., Higashidani, A., Tonozuka, T., Freeze, H.H., Nishikawa, A. (2008). The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells FEBS J.  . 275, 788-98.
  13. Park, Y., Zhang, Z., Laremore, T.N., Li, B., Sim, J.S., Im, A.R., Ahn, M.Y., Kim, Y.S., Linhardt, R.J., Glycoconj, J. (2008). Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatina fulica) Glycoconjugate Journal. 25, 863-77.
  14. Banala J. et al. (2008). Caged substrates for protein labeling and immobilization Chembiochem . 4, 38-41.
  15. Macnaughtan, M.A., Tian, F., Liu, S., Meng, L., Park, S., Azadi, P., Moremen, K.W., Prestegard, J.H. (2008). 13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I J. Am. Chem Soc. . 130, 11864-5.
  16. Oh, D.B., Park, J.S., Kim, M.W., Cheon, S.A., Kim, E.J., Moon, H.Y., Kwon, O., Rhee, S.K., Kang, H.A. (2008). Glycoengineering of the methylotrophic yeast Hansenula polymorpha for the production of glycoproteins with trimannosyl core N-glycan by blocking core oligosaccharide assembly Biotechnol. J. . 3, 659-68.
  17. Baudino L, Shinohara Y, Nimmerjahn F, Furukawa J, Nakata M, Martínez-Soria E, Petry F, Ravetch JV, Nishimura S, Izui S. (2008). Crucial role of aspartic acid at position 265 in the CH2 domain for murine IgG2a and IgG2b Fc-associated effector functions J Immunol. . 181(9), 6664-9.
  18. Teuton, J.R., Brandt, C.R. (2007). Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells  J. Virol. . 81, 3731-9.
  19. Aoki, K., Perlman, M., Lim, J.M., Cantu, R., Wells, L., Tiemeyer, M. (2007). Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo J. Biol. Chem. . 282, 9127-42.
  20. Song, Y., Choi, M.H., Park, J.N., Kim, M.W., Kim, E.J., Kang, H.A., Kim, J.Y. (2007). Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans Appl. Environ. Microbiol.  . 73, 4446-54.
  21. Sielaff I. et al. (2006). Protein function microarrays based on self-immobilizing and self-labeling fusion proteins ChemBioChem. . 7, 194-202.
  22. Eichler, R., Lenz, O., Garten, W., Strecker, T. (2006). The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein.GP-C. Virol. J. . 3, 41.
  23. Hagen, B.M., Sanders, K.M. (2006). Deglycosylation of the beta1-subunit of the BK channel changes its biophysical properties Am. J. Physiol.Cell. Physiol.  . 1, 750-6.
  24. O’Reilly, M.K., Zhang, G., Imperiali, B. (2006). In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis Biochemistry . 8, 9593-603.
  25. Srikrishna, G., Brive, L., Freeze, H.H. (2005). Novel carboxylated N-glycans contain oligosaccharide-linked glutamic acid Biochem. Biophys. Res. Commun. . 332, 1020-7.
  26. Cipollo, J.F., Awad, A.M., Costello, C.E., Hirschberg, C.B. (2005). N-Glycans of Caenorhabditis elegans are specific to developmental stages J. Biol. Chem. . 280, 26063-72.
  27. Lüftenegger, D., Picard-Maureau, M., Stanke, N., Rethwilm, A., Lindemann, D. (2005). Analysis and function of prototype foamy virus envelope N glycosylation J. Virol.  . 79, 7664-72.
  28. Whistler, J.L., Enquist, J., Marley, A., Fong, J., Gladher, F., Tsuruda, P., Murray, S.R., Von Zastrow, M. (2002). Modulation of postendocytic sorting of G protein-coupled receptors Science . 297, 615-20.
  29. Kalidas, C., Joshi, L., Batt, C. (2001). Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris Protein Eng. . 14, 201-7.
  30. Wong-Madden, S.T., Landry, D., and Guthrie, E.P. (1997). Discovery and Uses of Novel Glycosidases. In Techniques in Glycobiology Reid Townsend, Arland T. Hotchkiss . 401-408.