Note that Sample Purification beads are not included in this kit. If beads are required, we recommend ordering NEBNext® Ultra™ II FS DNA Library Prep with Sample Purification Beads (NEB #E6177).
See what others are saying about NEBNext Ultra II FS.
The NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content. The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, and the NEBNext Ultra II Q5® Master Mix for uniform, high-fidelity library amplification. Please note that adaptors and primers are not included in the kit and are available separately.
For protocols including bisulfite converted DNA, we recommend the NEBNext Ultra II DNA Library Prep Kit for Illumina.
Features:
Download extensive performance data in our technical note.
Also available with optional SPRIselect® beads for size selection/clean-up steps
For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.
View additional data presented at AGBT by Peter Ellis, Senior Staff Scientist at the Wellcome Trust Sanger Institute.
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