NEB® 5-alpha Competent E. coli (High Efficiency) | DH5α | NEB

Home Competent Cells Products NEB^® 5-alpha Competent E. coli (High Efficiency)

Catalog #	Concentration	Size	List Price	Your Price	Quantity
C2987H	Not Applicable	20 x 0.05 ml	$207.00	Sign In Sign In
C2987I	Not Applicable	6 x 0.2 ml	$159.00	Sign In Sign In
C2987P	Not Applicable	1 96-well plate (20 μl/well)	$523.00	Sign In Sign In
C2987R	Not Applicable	1 384-well plate (10 μl/well)	$1,144.00	Sign In Sign In
C2987U	Not Applicable	96 x 0.05 ml (12 8-well strip)	$778.00	Sign In Sign In

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Product Information

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications. Available in 5 convenient formats:

Single-use 50 μl vials
200 μl vials
96-well plate
384-well plate
12 x 8-tube strips: packed in 12 x 8-Strip Microplate Cluster Tube (Costar™ Cluster Tube System)

For C2987H, C2987I, C2987P and C2987U, SOC Outgrowth Medium is provided.
For C2987R, Outgrowth Medium 1.5 is provided.

Highlights

DH5α™ derivative
Free of animal products
Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA2)
Suitable for blue/white screening by α-complementation of the β-galactosidase gene
Reduced recombination of cloned DNA (recA1)
K12 Strain

Transformation Efficiency

For C2987H, C2987I, C2987P and C2987U: 1 - 3 x 10⁹ cfu/μg pUC19 DNA
For C2987R: 1 - 5 x 10⁸ cfu/μg pUC19 DNA

Genotype

fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

This product is related to the following categories:: Cloning Competent Cell Strains Products

This product can be used in the following applications:: USER^® Cloning,; Applications of USER® and Thermolabile USER II Enzymes,; Transformation,

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection	Working Concentration
Ampicillin	100 µg/ml
Carbenicillin	100 µg/ml
Chloramphenicol	33 µg/ml
Kanamycin	30 µg/ml
Streptomycin	25 µg/ml
Tetracycline	15 µg/ml

Shipping Notes

Ships on dry ice

Antibiotic Resistance

none

Protocols, Manuals & Usage
Tools & Resources
- Selection Charts
  Characteristics of Select E.coli Strains
  
  Competent Cell Product Comparison
  
  Competent Cell Selection Guide
- Web Tools
  Competitor Cross-Reference Tool
  
  NEBcloner^®
FAQs & Troubleshooting
- FAQs
  Which competent cell strains are compatible with Gateway^® Cloning?
  
  Can I store competent cells at -20°C instead of -80°C?
  
  How can I increase transformation efficiency?
  
  How long should I incubate cells on ice after DNA has been added (NEB #C2987H and NEB #C2987I)?
  
  How should I calculate the transformation efficiency (C2987)?
  
  What are the solutions/recipes (C2987)?
  
  What are the strain properties (C2987)?
  
  What is the difference between NEB #C2987H and NEB #C2987I?
  
  What is the difference between NEB #C2988J and NEB #C2987H?
  
  What is the optimal heat shock time for this strain (NEB #C2987H and NEB #C2987I)?
  
  What type of competent cells are suitable for transformation of DNA constructs created using Gibson Assembly?
  
  Which strain of Competent E.coli should I use for general cloning?
  
  Which kind of transformation tubes should be used?
  
  What volume of DNA can be added into competent cells?
  
  Are NEB's competent cells compatible with the “Mix & Go" protocol?
  
  What is the shelf life for this strain (NEB #C2987H and NEB #C2987I)?
  
  How does the transformation efficiency of the 96-well plate format (NEB #C2987P) compare to the other formats?
  
  What is the optimal heat shock time for the 96-well plate format NEB 5-alpha Competent E.coli (NEB #C2987P)?
  
  I am competing in the iGEM competition. Do you have any products that I should consider from NEB?
  
  What is the optimal heat shock time for the 384-well plate format NEB 5-alpha Competent E. coli (NEB #C2987R)?
  
  What is the optimal heat shock time for the 96-tube format NEB 5-alpha Competent E. coli (NEB #C2987U)?
  
  What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix?
  
  How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
  
  How should fragments be prepared for assembly using NEBuilder HiFi?
Citations & Technical Literature
- Citations
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
  
  Additional Citations
  
  Kostylev M., Otwell A.E., Richardson R., Suzuki Y. (2015) Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies PLoS One; 10, e0137466. PubMedID: PMC4562628, DOI: 10.1371/journal.pone.0137466
  
  Lee S.B., Park S.K., Kim Y.S. (2015) Production of bioactive chicken (Gallus gallus) follistatin-type proteins in E. coli Sci Rep; 5, 11603. PubMedID: 26302688, DOI: 10.1038/srep11603
  
  Mascher G, Derman Y, Kirk DG, Palonen E, Lindström M, Korkeala H (2014) The CLO3403/CLO3404 Two-Component System of Clostridium botulinum E1 Beluga Is Important for Cold Shock Response and Growth at Low Temperatures Appl Environ Microbiol; 80(1), 399-407. PubMedID: 24185852, DOI: 10.1128/AEM.03204-13

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