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ERCC transcript correlation
Nondir_RNA_Complexity_Figure2
Poly(A)-containing mRNA was isolated from Human Universal Reference RNA (Agilent #740000) with recommended amounts of ERCC RNA Spike-In Mix I (Thermo Fisher Scientific #4456740) and libraries were prepared using the NEBNext Ultra II RNA Kit (plus the NEBNext Poly(A) mRNA Magnetic Isolation Module), and the Illumina TruSeq RNA Sample Preparation Kit v2. Salmon 0.6 was used for read mapping and quantification of all GENCODE v25 transcripts. TPM = Transcript Per Kilobase Million. R2 values for the linear fit are shown. Correlation analysis of the transcripts indicates very good transcript expression correlation between the different input amounts for ERCC spike-ins. The curved fit is a consequence of log transformation.

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