FAQ: What other materials do I need in addition to the NEBNext Ultra II First Strand Synthesis Module for RNA-seq experiments?

If you are starting from total RNA, we recommend either the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) or the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400 or NEB #7405) or NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E7750 or NEB #E7755) or NEBNext® rRNA Depletion Kit (Bacteria) (NEB #E7850 or NEB #E7860) or NEBNext® RNA Depletion Core Reagent Set (NEB #E7865 or NEB #E7870) or NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310 or NEB #E6350) for use prior to first strand synthesis.

For strand-specific RNA-seq workflows, use the following kits:
NEBNext Ultra II Directional RNA Second Strand Synthesis Module (NEB #E7550)
NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
NEBNext Ultra II Ligation Module (NEB #E7595)
NEBNext Ultra II Q5 Master Mix (NEB #M0544)

For non-directional RNA-seq workflows, use the following kits:
NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB #E6111)
NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
NEBNext Ultra II Ligation Module (NEB #E7595)
NEBNext Ultra II Q5 Master Mix (NEB #M0544)

For adaptors and primers, NEBNext Oligos are compatible, as are adaptors and primers from other sources.