Home FAQs What is the difference between the NEBNext Ultra II RNA Library Prep with Sample Purification Beads (NEB #E7775) and the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB #E7765)?

FAQ: What is the difference between the NEBNext Ultra II RNA Library Prep with Sample Purification Beads (NEB #E7775) and the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB #E7765)?

The NEBNext Ultra II Directional RNA Library Prep workflow preserves information about RNA strand orientation while the NEBNext Ultra II RNA Library Prep workflow does not. The NEBNext Ultra II Directional RNA Library Prep contains dUTP in the second strand synthesis buffer that allows labeling of the second strand cDNA and subsequent excision with USER Enzyme.