FAQ: What is the difference between using Colorimetric LAMP and regular LAMP reactions?

Our pH-based colorimetric LAMP technology is based on the same principle of LAMP and RT-LAMP amplification, but has been designed to allow visual detection of positive reactions. The WarmStart Colorimetric LAMP 2X Master Mixes contains the pH-sensitive dye Phenol Red in a low-buffer reaction solution that changes color from bright pink to yellow upon DNA amplification (due to the release of a proton per nucleotide incorporated). This detection makes it possible to use simple equipment (e.g., hot water for incubation, visual detection) instead of sophisticated and expensive instruments for LAMP reactions.